The effects of mite parasitism on the reproduction and survival of the Taiwan field mice (Apodemus semotus)
© Lin et al.; licensee BioMed Central. 2014
Received: 23 May 2014
Accepted: 21 November 2014
Published: 17 December 2014
The effects of parasitism on host survival and reproduction can be highly complex depending on the type of parasites, host sex and life-history characteristics, and ecological conditions. In this study, we tested sex-biased parasitism from Trombiculid mites (Acarina: Trombiculidae) and their sex-specific effects on host reproduction and survival, in a natural population of the Taiwan field mouse (Apodemus semotus). We performed surveys of A. semotus and their Trombiculid mites between April 2010 and August 2011 and again between June and September 2012 in a subtropical evergreen forest in Taiwan.
Contrary to the commonly reported male-biased parasitism in mammals, we did not find sex-biased parasitism in A. semotus. We found that mite abundance was negatively associated with A. semotus reproduction and survival in both males and females. The mite abundance and rodent reproduction fluctuated seasonally, and the peak reproductive season coincided with the time period of relatively low mite abundance.
Trombiculid mites could potentially regulate A. semotus populations through reducing their reproduction and survival. The overlapping periods of peak reproduction and low parasitism implied that A. semotus may adjust their reproductive phenology to avoid periods of high parasitism or be constrained by parasites to reproduce only during periods of low parasitism. Although our results are correlational, host breeding season has been shown to increase in response to experimental reduction of parasitism. We suggest that parasites may shape host reproduction phenology through which they may influence host population dynamics.
Parasites can alter host physiology (Schall et al. ; Simon et al. ), body condition (Hakkarainen et al. ; Vandegrift et al. ; Turner et al. ), behavior (Schall et al. ; Goodman and Johnson ), social dominance (Schall and Houle ; Zohar and Holmes ), and life history (Schall ; Schall and Dearing ; Shields and Wood ; Merino and Potti ; Lochmiller and Deerenberg ; Skarstein et al. ; Chadwick and Little ; Cox and John-Alder ; Kallio et al. ), leading to complex ecological and evolutionary consequences in host populations (Hamilton and Zuk ; Buckling and Rainey ; Chadwick and Little ). Theoretical and field studies suggest that parasites can regulate host populations, playing a role comparable to predators (May and Anderson ; Albon et al. ; Redpath et al. ; Lemaître et al. ). Unlike predator-prey interactions where survival is arguably the single most important parameter to consider, parasites may affect host reproduction, survival, or the trade-off between the two (Sheldon and Verhulst ; Stien et al. ; Burns et al. ; Vandegrift et al. ; Lemaître et al. ), making their role in host regulation an extremely dynamic phenomenon.
Reduction in host fecundity is commonly found in parasitic associations (Hurd ). For example, ectoparasite removal increased litter size in Columbian ground squirrels (Spermophilus columbianus) (Neuhaus ), as well as juvenile mass at emergence and their subsequent survival probabilities in North American red squirrels (Tamiasciurus hudsonicus) (Patterson et al. ). A similar effect was also found in endoparasites. Vandegrift et al. () demonstrated that helminth parasites prevented the white-footed mouse (Peromyscus leucopus) from breeding in mid-summer. However, positive associations between parasitism and host fecundity that resulted from immunosuppression or behavioral change caused by sex hormone during breeding season were also reported (Schwanz ; Gooderham and Schulte-Hostedde ). A recent study on red squirrels (T. hudsonicus) demonstrated that helminth parasites were negatively associated with reproductive success in both male and female squirrels whereas fleas and mites were positively associated with male reproductive success (Gooderham and Schulte-Hostedde ). Therefore, the relationship between parasitism and host reproduction may vary depending on the life histories of the host and parasite species.
Sex-biased parasitism is common in natural host populations (Poulin ; Zuk and McKean ; Schalk and Forbes ; Moore and Wilson ; Klein ; Morand et al. ; Cox and John-Alder ; Krasnov et al. ). Among vertebrates, parasitism risk is generally higher for males due to a presumed trade-off between sexual traits and parasite defense (Poulin ; Zuk and McKean ; Schalk and Forbes ; Morand et al. ) or behaviors that incur high infection rates (e.g. defending territories, aggression; Brown et al. ). Nevertheless, female-biased parasitism and lack of sex-biased parasitism in small mammals have also been reported (Morales-Montor et al. ; Christe et al. ; Sanchez et al. ). In species for which nutritional needs during reproduction compromise mothers’ immunity (Klein and Roberts ) or encourage mothers to engage in behaviors with increased infection risks (e.g. feeding at contaminated food patches, overlapping with others’ home ranges; Sanchez et al. ), the trade-off between reproduction and parasite defense can be stronger for females or becomes equal between the sexes. In addition to sex-biased parasitism, parasitism effects on hosts could also be sex-specific (Vandegrift et al. ; Gooderham and Schulte-Hostedde ; Bordes et al. ). For example, helminth removal increased breeding only in female P. leucopus and survival only in the males (Vandegrift et al. ). Therefore, more empirical evidence is needed to fully understand the causes and consequences of sex-biased parasitism and sex-specific parasitism effects in natural populations (Krasnov et al. ; Bordes et al. ).
Recent meta-analyses reported a latitudinal gradient in parasite-induced mortality, with parasite virulence and associated host mortality increasing toward lower latitudes (Møller et al. ; Robar et al. ). However, ecological studies of parasitism in subtropical/tropical regions remain relatively rare (Waller ). In this study, we examined sex-biased parasitism from Trombiculid mites (Acarina: Trombiculidae) and their sex-specific effects on host reproduction and survival, in a natural population of the Taiwan field mouse (Apodemus semotus) in a subtropical forest. Trombiculid mites, a main vector of Orientia tsutsugamushi that causes scrub typhus in human, are parasitic at larval stage and can be found on a wide range of vertebrate hosts including birds, mammals, and lizards (Nelson et al. ). Trombiculid larvae (chiggers) attach to a host where the skin is thin for feeding, which usually lasts several days to a few weeks (Wharton and Fuller ; Baker et al. ). Free-living stage of Trombiculid mites are soil dwellers with complex life cycles (reviewed by Shatrov and Kudryashova ). Although seasonal patterns of mite development are still unclear, available reports suggest that these mites could retard their development at any stage in the cold period (e.g. temperature <10°C; Takahashi et al. ). In the tropics, these mites may continue their development without any conspicuous diapause and have a much shortened life cycle (reviewed by Shatrov and Kudryashova ). For example, under laboratory conditions (i.e., 27°C and relatively humidity of 85%), the entire life cycle of Trombiculid mites can be completed in 2 to 3 months (Traub and Wisseman ), as appose to 150 to 400 days among boreal species (Shatrov and Kudryashova ). Therefore, seasonality in their larval emergence and abundance is likely influenced by the climate of the study system. Apodemus is the most common rodent in forests of the Palaearctic region with over 20 species (Corbet ; Corbet and Hill ), many of which are known hosts to Trombiculid mites (Ree et al. ; Xing-Yuan et al. ). Therefore, the ‘Apodemus-Trombiculid mites’ association provides an ideal system to study parasite effects on host.
Using an observational data set, we asked the following questions: (1) Is mite parasitism sex-biased? (2) Is mite parasitism related to host body mass? (3) Is mite parasitism associated with host survival and reproduction? (4) Do the associations in (3) sex-specific?
The study system
The study was conducted in an evergreen forest (Pinus-Alnus-Quercus) in the Shei-Pa National Park (121°18′ E, 24°21′ N; elevation c. 1,800 m). The climate at the study site can be grouped into three seasons, the warm and moist spring (March through June, mean temperature: 14°C, relative humidity: 88%, precipitation: 835 mm), the warm and wet summer (July through October: mean temperature: 16°C, relative humidity: 87%, precipitation: 2,059 mm), and the cool and dry winter (November through February, mean temperature: 9°C, relative humidity: 85%, precipitation: 387 mm) (2005 to 2008 weather data, Taiwan Forestry Bureau). The information on mite taxonomy is scarce for this geographic region. However, we identified two genera of Trombiculid mites, Leptotrombidium spp. and Walchia spp., which use A. semotus as hosts.
The trapping was performed from April 2010 to August 2011 (the 2010 to 2011 survey) and again from June to September 2012 (the 2012 survey). During the 2010 to 2011 survey, we established three trapping grids, each comprising 64 stations arranged in an 8 × 8 array (10 m between stations). The distance between adjacent grids was c. 60 m apart. This distance was chosen based on the average distance moved by adult A. semotus between two successive days (c. 17 to 34 m) and their average home range size (c. 1,267 to 3,270 m2, equivalent to 36 to 57 m in radius) reported in Lin and Shiraishi ([1992a]). One live trap was set up at each trapping station in the evening (192 traps per night across the three grids), baited with sweet potato, mixed seeds, and peanut butter, and checked the next morning. A total of nine trapping sessions were performed (April, June, August, October, and December 2010 and February, April, June, and August 2011), each comprising three consecutive days. During the 2012 survey, we established one trapping grid at the same site, comprising 36 stations arranged in a 6 × 6 array (10 m between stations). Two live traps were set up at each trapping station in the evening (72 traps per night), baited with sweet potato, mixed seeds, and peanut butter, and checked the next morning. A total of four monthly trapping sessions from June to September were performed. Due to weather constraints, we performed the trapping for five consecutive days in June and September, but only for three consecutive days in July and August.
Upon capture, each rodent was marked with an ear tag (National Band and Tag Co., Newport, KY, USA; during the 2010 to 2011 survey) or a radio-frequency identification chip (Watron Technology Corp., Hsinchu, Taiwan; during the 2012 survey), sexed, and weighted. Their physical signs of reproduction were also recorded, including descended testicles in males, pregnancy, lactation, and perforated vagina in females. For males, descended testicles indicate that they are reproductively capable, whereas for females, pregnancy and lactation indicate breeding, and perforated vagina indicates mating. Therefore, these physical signs are more direct indicators of reproduction in females than in males. The mice were immediately released after processing at the station where they were captured. All procedures in mice trapping and sampling were approved by the National Taiwan Normal University’s Animal Care and Use Committee (protocol no. 102004).
The lifespan of A. semotus in the wild is approximately 1 year. At the current study site, we had previously reported that only 1% to 10% of the individuals were recaptured beyond 2 months (based on data from August 2010 to June 2011 and from May to September 2013; Shaner et al. ). Therefore, we estimated rodent survival as the probability of surviving a 2-month period using the Cormack-Jolly-Seber (CJS) model (White and Burnham ). The CJS model estimates apparent survival (φ) and recapture probability (p). Apparent survival is the probability that an individual had survived a given time period and did not emigrate out of the study area, and recapture probability is the probability that an individual had survived a given time period and was recaptured at the next trapping period. One advantage of the CJS model is that survival and recapture probabilities can be simultaneously estimated as functions of individual covariates (e.g. parasite abundance on a rodent). If parasites affect host activity, individuals with different parasite abundances may have different recapture probabilities, potentially confounding the effect of parasites on host survival. By incorporating parasite abundance as an individual covariate for φ and/or p, we can control for parasite effect on host recapture probability while evaluating their effect on host survival.
where βfemale and βmale are female- and male-specific slope of mite abundance for the survival function, and intfemale and intmale are female- and male-specific intercept for the survival function. When βfemale = βmale and intfemale = intmale, the model of sex-specific φ reduces to constant φ. βp and intp are the slope and intercept for the recapture probability function. Mite abundance is based on the number of mites found on each individual, grouped into four levels: ‘0’ (zero mite), ‘1’ (1 to 9 mites), ‘2’ (10 to 19 mites), and ‘3’ (20 mites or more) (see ‘Mite infection and abundance’ below). For repeatedly captured individuals, the mean level of mite abundance is used.
Mite infection and abundance
Mite data were collected between December 2010 and August 2011 (the last five trapping session in the 2010 to 2011 survey) and again between June and September 2012 (all four trapping sessions in the 2012 survey). During the 2010 to 2011 survey, we temporarily restrained each rodent by hand to inspect the mites attached to the ears (Additional file 1: Figure S1) and the skin area around the genital region. We grouped the number of mites found on each individual into one of the four levels: ‘0’ (zero mite), ‘1’ (1 to 9 mites), ‘2’ (10 to 19 mites), and ‘3’ (20 mites or more). The lowest level of zero mite found indicates uninfected animals (mite infection = 0), whereas the other three levels indicate infected animals (mite infection = 1). Although this method only provides an ordinal measure of mite abundance, it does not require placing the animals under anesthesia, for which we did not have adequate logistic support at the time. During the 2012 survey, however, we were able to perform anesthesia (2% to 4% isoflurane administered through a gas anesthesia system with a nose cone, AN-10 T, Singa Technology Corp., Taipei, Taiwan) to obtain a complete count of the mites. The 2012 data set indicates that the mite counts were positively correlated with the four levels of mite abundance (r = 0.57; P = 0.0007; n = 32; Additional file 1: Figure S1). Furthermore, 91% of the mice in the 2012 data set had a mite count between 0 to 29 (0 mite, 1 to 9 mites, 10 to 19 mites, 20 to 29 mites; Additional file 1: Figure S1), suggesting that the four abundance levels can effectively capture most of the variation in mite count with limited data lumping.
The nine trapping sessions for which we had parasitism data were grouped into three seasons according to the climate at the site: spring (April and June 2011, June 2012), summer (August 2011, July, August, and September 2012), and winter (December 2010 and February 2011). We tested the effects of sex, season, and their interactions on mite parasitism using the generalized linear mixed model (GLMM). The response variable is mite infection (0/1) or mite abundance (zero mite, 1 to 9 mites, 10 to 19 mites, 20 mites or more). When testing mite infection, we applied binomial distribution with logit link function; when testing mite abundance, we applied Poisson distribution with log link function. The survey year, trapping grid, and individual identity were included as random factors to account for spatial-temporal heterogeneity and non-independence of repeated records from the same individuals. Although none of the mice were captured in both years, ten individuals (5% of the 214 unique individuals) were observed on more than one grid. We assigned different grid codes to these cross-grid individuals (e.g. the three mice that were observed on both grids A and B were assigned ‘AB’ as their grid value). To check if the effects of sex, season, and their interactions on mite abundance are sensitive to our four-level grouping of mite abundance, we also performed GLMM on mite counts (Poisson distribution) using only the 2012 data. Because only one trapping grid was used in 2012, we dropped trapping grid and survey year from the random factors (individual identity was kept as a random factor). We tested the effects of sex, season, reproduction, mite abundance, and their interactions on A. semotus body mass using GLMM (normal distribution). We tested the effects of sex, season, mite abundance, and their interactions on A. semotus reproduction using GLMM (binomial distribution). For the body mass and reproduction models, we included trapping grid, survey year, and individual identity as random factors. To reach model convergence for the reproduction analysis, we reduced the four levels of mite abundance to 3 (0 to 9 mites, 10 to 19 mites, 20 or more mites). Type I errors for post hoc comparisons following the GLMMs were Tukey-Kramer adjusted. To test parasitism effect on A. semotus survival, we obtained the 95% confidence limits of the slopes of mite abundance on survival (φ) from the CJS model. An upper 95% confidence limit smaller than zero indicates a negative association between mite abundance and rodent survival whereas a lower 95% confidence limit greater than zero indicates a positive association. All statistical analyses were performed in SAS 9.3.
Reproduction phenology and population abundance of the host
Sex and seasonal difference in mite parasitism
The generalized linear mixed model of the infection and abundance of Trombiculid mites in Apodemus semotus
Sex × season
Data on host body mass and reproduction state were available for all 291 records of mite parasitism from 214 unique adults (78 females and 136 males). Of these 214 adults, we obtained capture-recapture histories from 189 unique individuals (66 females and 123 males) from the 2010 to 2011 survey for survival estimates.
The generalized linear mixed model of Apodemus semotus body mass
Sex × season
Sex × reproduction
Sex × mite abundance
Season × reproduction
Season × mite abundance
Reproduction × mite abundance
Sex × season × reproduction
Sex × season × mite abundance
Sex × reproduction × mite abundance
Season × reproduction × mite abundance
Sex × season × reproduction × mite abundance
Host body mass, reproduction, and survival
The generalized linear mixed model of Apodemus semotus reproduction
Sex × season
Sex × mite abundance
Season × mite abundance
The Cormack-Jolly-Seber model of Apodemus semotus survival and recapture probability as functions of mite abundance
Recapture probability function
−1.50 (−3.21, 0.22)
9.33 (1.73, 16.94)
0.42 (−0.84, 1.68)
−0.73 (−1.47, 0.02)
0.42 (−0.39, 1.22)
−0.58 (−1.01, −0.16)
0.39 (−0.30, 1.07)
−0.61 (−0.98, −0.24)
−1.47 (−3.20, 0.25)
9.31 (1.70, 12.92)
In this study, we showed that parasitism from Trombiculid mites was associated with reduced reproduction and survival in a wild rodent, A. semotus, suggesting a potential role of Trombiculid mites in regulating their host populations. This negative association was similar between male and female A. semotus. Furthermore, we did not find sex-biased parasitism in either breeding or non-breeding season. This is one of the few empirical studies on host-parasite ecology from a relatively under-studied geographic region (Southeast Asia), where warm and wet climates year-round may allow ectoparasites with free-living stages to breed more frequently and persist at high densities (Takahashi et al. ; Waller ; Shatrov and Kudryashova ).
Sex-biased parasitism is a complex phenomenon that can be influenced by the biology of particular host and parasite species, mediated by environmental factors, and exhibited spatial and temporal variation (reviewed by Krasnov et al. ). Although ectoparasites tend to show male-biased parasitism (Krasnov et al.  and references therein), several ecological characteristics of Trombiculid mites and A. semotus may help explain the lack of sex-biased parasitism in this study. Trombiculid mites only remain attached to a host individual for a short period of time (several days to a few weeks; Wharton and Fuller ; Baker et al. ), and they appear to be tightly associated with particular habitats in which they are able to infect a wide range of host species (Shatrov and Kudryashova ), suggesting this parasite may not be choosy about host individuals. A recent study on Trombiculid mites (Leptotrombidium imphalum) in lowland Taiwan suggests that they are not able to freely distribute themselves according to the quality of host individuals, presumably due to their limited mobility (Kuo et al. ). Host sexual dimorphism in spacing behavior, immune defense, and/or body size can work synergistically or individually to drive sex-biased parasitism. Although male A. semotus are larger than females, the difference in their body mass is less than 2 g (c. 7% of the body mass). Furthermore, male and female A. semotus have similar home-range sizes, and their home ranges often overlap (Lin and Shiraishi [1992a]). To the best of our knowledge, sexual dimorphism in immune defense in A. semotus has not been studied. However, studies on other rodents suggest that host gender may not have a systematic effect on their immune defense (Khokhlova et al. ; Waterman et al. ). Therefore, the ecology of A. semotus does not seem to support sex-biased parasitism.
If these mites have limited ability to select and switch between host individuals, their infection and abundance should be primarily determined by host spacing behavior. Following this argument, the negative association between mite parasitism and the survival and reproduction of A. semotus could be caused by different spacing behaviors in the host. For example, reproducing or long-lived mice may have smaller or more stable home ranges, reducing their encounter rates with the parasites. However, based on a study of A. semotus home ranges at a different site (Lin and Shiraishi [1992a]), their length of capture history (a proxy for survival) was not correlated with their home range sizes. Unfortunately, the trapping protocol in the current study is spatially and temporally too sparse to determine their home ranges. Therefore, spacing behaviors of reproducing versus non-reproducing A. semotus, as well as A. semotus with different life-history strategies (long-lived versus short-lived), remained to be studied.
Although characteristics of host individuals, such as body mass and age, are frequently documented to influence patterns of parasitism, we did not find a relationship between mite abundance and host body mass, regardless of host sex, host reproductive state, or season. The lack of relationship between host body mass and mite parasitism, however, suggests that any potential parasitism effect on A. semotus survival or reproduction is not likely mediated through their body mass. On the other hand, if older hosts are more likely to reproduce yet less likely to be parasitized (either because of their lower quality as hosts or their more effective parasite defense), the negative association between mite parasitism and A. semotus reproduction could reflect an age effect. Although majority of the mice in this study were only captured once, we were able to examine 36 individuals (24 males and 12 females) that were known to have survived at least 4 months as adults from the 2010 to 2011 survey (adults are usually more than 1 month old; Lin and Shiraishi [1992b]; PLS, personal observation). We did not find an age effect on individuals’ probability of reproduction for either sex (Additional file 1: Table S1). In fact, one adult male with eight consecutive recaptures spanning a total period of 14 months (June 2010 to August 2011) showed a reproduction record of 1 1 0 0 1 0 1 1, illustrating that their reproduction is quite flexible and may be adjusted according to individual and environmental conditions.
Parasitism from Trombiculid larvae has been reported to vary seasonally (Klukowski ; Dietsch ). Seasonality in parasitic infection can be explained by parasite-related and/or host-related traits. Under laboratory conditions (i.e., 27°C and relatively humidity of 85%), the entire life cycle of Trombiculid mites can be completed in 2 to 3 months (Traub and Wisseman ). Our study site is in a subtropical montane forest with warm and humid climate year-round, which may help explain the high prevalence of mite parasitism across seasons (0.70 to 0.94; Figure 2a). Against the high background level of mite prevalence, we also found seasonal variation in their prevalence and abundance. Interestingly, the season of lower mite abundance coincided with peak reproductive season for the host, suggesting A. semotus might be adjusting their reproduction phenology to avoid parasitism, or they might be constrained to reproduce during periods of low parasitism. Although this off-set in timing between host reproduction and increased parasitism risk could be coincidental, we know of at least one study that experimentally demonstrated it. Vandegrift et al. () showed that another rodent host, P. leucopus, was able to extend their breeding season to mid-summer when their helminth parasites were experimentally reduced. In fact, many host-related traits (e.g. territoriality, immunocompetence, hormone) and their physiological trade-offs have been shown to vary between breeding and non-breeding seasons (reviewed by Krasnov et al. ; Martin et al. ), contributing to seasonal fluctuation in parasitism. Rodent species have been reported to invest less in immune defense if probability and/or intensity of parasite attack is low and vice versa (Khokhlova et al. ; de Bellocq et al. ). Consequently, if immune defense is costly for A. semotus, which is a likely scenario considering the consistently high prevalence of mite parasitism they are facing (Khokhlova et al. ; de Bellocq et al. ), it would be more advantageous, or it would be only possible, for these mice to breed during periods of low probability and/or intensity of parasitism.
During this study, we were able to record the presence of ticks (Ixodes) on A. semotus. Of the 235 rodents that we examined for ticks, we documented 23 cases of infection and all of them occurred in the winter between December and February (PLS, unpublished results). Although tick infection appeared to be rare (10%) and cannot be subjected to further quantitative analysis, it suggests that winter months at this study site may provide favorable conditions for ectoparasites with non-parasitic life stages. Therefore, it is important to study the life cycles of parasites in the wild before we can fully understand host-parasite dynamics.
In this study, we showed that mite parasitism in a rodent host was associated with reduced host reproduction and survival, with implications in the regulation of host populations and evolution of host life history. Our study is one of the few empirical studies of host-parasite ecology from a subtropical region where climate conditions are favorable for many parasites to persist at high abundance. Such empirical evidence adds valuable information to our overall understanding of the general rules governing host-parasite dynamics.
We thank Si-Min Lin and Chi-Chien Kuo for insightful discussions on parasite ecology that inspired current study; Linghua Ke and Cong-Kun Zhou for their assistance in field work; and the Shei-Pa National Park for providing logistic support. This work was funded by the National Science Council in Taiwan (NSC 99-2621-B-003-001 and NSC 100-2621-B-003-006).
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