Genetic analysis of parthenogenetic capability and fecundity in Drosophila albomicans
© Chang and Chang; licensee Springer. 2014
Received: 25 February 2014
Accepted: 11 June 2014
Published: 3 July 2014
The successful rate of parthenogenesis in Drosophila harvested from natural population was extremely low, which could be effectively improved under selection pressure. Facultative parthenogenesis in Drosophila albomicans may be advantageous for its expansion from sub-tropical to temperate area. Since the understanding of the genetics involved in the capability and fecundity of parthenogenesis is limited, this study aims to preliminarily map the chromosome regions that are preferentially important for parthenogenesis.
Genetic mapping was performed with F2 individuals that were parthenogenetically produced by F1 from crosses between a parthenogenetic strain KKU119 and a sexual strain #55.1 of Drosophila albomicans. Among 105 F2, 53.3% of them had parthenogenetic capability which is highly associated with three markers a28, c4081, and c7198 located near or inside In(2L)B 1 D 5 . A sexual strain with high In(2L)B 1 D 5 heterozygosity originating from Wulai, Taiwan in 1970 was able to perform parthenogenesis. However, the fecundity of those F2 varied in a wide range, forming a continuous distribution as expectation of a quantitative trait and was correlated with the number of homozygous markers for all markers on the second chromosome and neo-X chromosome arm.
We have genetically analyzed the capability and fecundity of parthenogenesis in Drosophila albomicans. The former is specifically associated with a limited region in the B1 to D5 of 2L arm where inversion In(2L)B 1 D 5 may play certain role for the maintenance of parthenogenesis, whereas the latter is apparently related to several quantitative loci on the second chromosome and neo-X chromosome arm.
Hundreds of lineages in the animal kingdom have experienced the evolution from sexual reproduction to parthenogenesis by which females reproduce offspring without mating (Schwander et al. ). As compared to sexual reproduction, the advantages of parthenogenesis include theoretically twofold fecundity, given all else is equal (Maynard Smith ), and increased potential of colonization in harsh, high latitude and/or altitude, isolated environments or newly invaded marginal habitats, where population size is usually too small for females to find mates (Suomalainen ). Alternatively, parthenogenesis may suffer disadvantages for long-term evolution because of the loss of genetic variation and accumulation of deleterious mutations (Simon et al. ). Facultative parthenogenesis, coexistence of parthenogenesis and sexual reproduction, is apparently the better reproductive strategy than parthenogenesis (Hurst and Peck ).
Most parthenogenetic Drosophila species conduct facultative parthenogenesis (Templeton ), and hence, they may escape from the accumulation of deleterious mutations and display higher fitness in the condition of low population density. Although the hatchability of unfertilized eggs of parthenogenetic Drosophila harvested from natural population is extremely low, it could be significantly increased under certain selection pressure (Carson ; Stalker ; Markow ). Since productivities were increased with generations under artificial selection, parthenogenesis is considered to be regulated by polygenes. However, if parthenogenesis is controlled by a polygenic system, successful parthenogens will be hard to evolve (Fuyama ). A study on the parthenogenetic Drosophila mercatorum reported that the genotypes for incapability of parthenogenesis were quickly eliminated in the first few generations, and the population size varied among different genotypes after several generations of parthenogenesis (Annest and Templeton ). Therefore, we consider that there are only a few essential genes for parthenogenetic capability while there are many genes influencing the fecundity (i.e., the number of parthenogenetically produced offspring). The former phenotype is either capable or incapable to perform parthenogenesis, and the latter indicates the parthenogenetic efficiency of genetically different females. Genetic mapping for parthenogenesis conducted on Drosophila ananassae complex with parthenogenetic capability as phenotype indicated a major gene located on its 2L chromosome arm (Matsuda and Tobari ). Fuyama () indicated that Drosophila melanogaster can perform gynogenetic reproduction (i.e., females can reproduce by mating with sterile males), which was related to recessive factors located between Tft locus and nw locus on 2R chromosome arm and Gl and Sb on 3R chromosome arm.
In the present study, we used Drosophila albomicans, a species invading from sub-tropical areas (i.e., probably Wulai, Taiwan) to the temperate climate zone (Ohsako et al. ), as material for genetic mapping. Parthenogenesis of Drosophila albomicans was first reported by Ohsako and Fuyama () in Kiikatsuura, Japan which was the boundary of Drosophila albomicans distribution. Strong natural selection in favor of parthenogenesis may be operated for an adaptation to a cold climate in which population density is low. Parthenogenetic strain was established with 80% to 90% of them capable of parthenogenesis and about 11 offspring were reproduced per female from 11th to 30th generation after strain establishment (Ohsako and Fuyama ). In order to find a proper sexual strain to cross with this parthenogenetic strain, we checked the parthenogenetic capability of strains. We crossed between the sexual strain and parthenogenetic strain and collected F1 virgin females to produce recombinant F2 since meiosis was performed during parthenogenesis. Therefore, the F2 offspring were adopted to map the genetic elements for parthenogenetic capability and do genetic analysis for parthenogenetic fecundity. We also discussed the maintenance of parthenogenesis in Drosophila albomicans.
The parthenogenetic Drosophila albomicans strain was provided as a courtesy from Dr. Fuyama. It was established from a virgin female of an isofemale strain (KKU119), which was originally collected from Kiikatsuura, Japan in 1990, and maintained by parthenogenesis ever since (Ohsako and Fuyama ). Isofemale sexual strains #55.1 and #56.1 were established in 1970 from Hualien and Wulai, Taiwan, respectively. For the chromosomal inversion arrangements, there is no inversion heterozygote on the neo-sex arm or the 2R arm in all of these three strains but only one inversion type, In(2L)B 1 D 5 , on the 2L arm. The strain #56.1 has about 75% In(2L)B 1 D 5 heterozygosity. The 2L arms of KKU119 and #55.1 are homozygous, but they carry different arrangements. Flies were maintained on standard cornmeal medium at 23°C under 12:12 h LD cycle. Flies used for crosses were sexed within 8 h after eclosion.
In order to do proper genetic mapping, we made sure that the chosen sexual strain could not perform parthenogenesis. The parthenogenetic capability of strains #55.1 and #56.1 was examined by transferring virgin females to new vials twice a week until 4 weeks. The vials were checked for offspring reproduced in 4 weeks.
Marker list with location, primer name, primer sequence, PCR annealing temperature, and restriction enzyme
Annealing temperature (°C)
Chang et al. ()
neo-X chromosome arm
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The association between genotypes and parthenogenetic capability was revealed by Fisher's exact test. Linkage disequilibrium D′ (Lewontin ) values among highly associated markers were calculated to reveal hitchhiking. When the linkage disequilibrium was high, we checked recombinant individuals through Fisher's exact test in order to reveal which of the two markers was more influential. Recombination rates for all markers were also calculated in order to estimate the unknown locations of markers. In addition, the correlation between the parthenogenetic fecundity and the number of KKU119 homozygous markers carried by F2 was done by the analysis of variance (ANOVA) test, and jackknifing was also performed to figure out whether or not there is any key marker for the fecundity.
Association between markers linked to In(2L)B 1 D 5 and parthenogenetic capability
Recombination rate (%) of markers on the second chromosome and neo-X chromosome arm
Recombination rate (%)
neo-X chromosome arm
Linkage disequilibrium ( D ′) for markers shown to be highly associated with parthenogenetic capability
Number of recombinant F 2 categorized into genotypes and whether or not produced F 3 offspring parthenogenetically
Pair of markers with recombination
Number of F2
The distribution of quantitative parthenogenetic fecundity loci
The facultative parthenogenesis, occasional parthenogenesis with primarily sexual reproduction, is not unusual in insect. It is found in at least ten insect orders (Simon et al. ). The success of parthenogenesis is in general dependent on parthenogenetic capability as well as fecundity. In the present study, we performed genetic analysis using 14 molecular markers to reveal that parthenogenetic capability is associated with a limited number of gene loci on 2L chromosome, whereas parthenogenetic fecundity is influenced by numerous quantitative loci distributed on whole genome.
In order to locate the gene for parthenogenetic capability, sexual strains were checked for their parthenogenetic capability. Since females from #55.1 did not perform parthenogenesis, this strain was chosen to cross with KKU119, and their F2 offspring parthenogenetically produced by F1 were used for the genetic analysis of parthenogenesis. An advantage of adopting this strain is that their parthenogenetic capability and fecundity must be contributed by KKU119, which has been cultured for 370 generations by parthenogenesis. Their parthenogenetic capability gene(s) should have high or even fixed frequency. Although crossing between strains #55.1 and KKU119 produces heterozygous In(2L)B 1 D 5 F1 and invalidates mapping on a portion of the 2L arm, other chromosome regions covered by scattered markers are not influenced. After crossing, all sexually reproduced F1 have the same genotypes, but their parthenogenetic F2 may have different genotypes via recombination. Therefore, those F2 were subjected to investigate the association between genotypes and parthenogenetic capability.
About half of recombinant homozygous F2 were unable to do parthenogenesis, which corresponds to the about half which did not acquire the capability allele from the parthenogenetic strain (i.e., ranging from 39% to 56% for 14 markers). On the contrary, the other half of them able to do parthenogenesis may carry the parthenogenetic allele. These results implicate that there is only one genetic element responsible for parthenogenetic capability. Among all markers, the three markers, a28, c4081, and c7198, were most strongly associated with parthenogenetic capability. However, due to crossing-over inhibition of the heterozygous In(2L)B 1 D 5 , they were treated as one genetic element contributing to parthenogenesis. In other words, the capability gene may be located very close to In(2L)B 1 D 5 . The only marker on the second chromosome, A185D, which did not associate with parthenogenesis is located over 50 cM apart from the other six markers therefore assorted independently just like markers on neo-X chromosome arm. To sum up, we infer that the parthenogenetic capability gene or gene cluster is located on 2L arm and is strongly associated with In(2L)B 1 D 5 . However, due to suppressed recombination rates on 2L arm in heterozygous F1, the precise location of the major gene(s) was difficult to be mapped by current genetic analysis. To that purpose, it is essential to find another sexual strain which is unable to perform parthenogenesis while carries the same chromosome arrangement of 2L as that of the parthenogenetic strain for detailed mapping of the major gene(s).
Since the major gene or gene cluster is highly linked to In(2L)B 1 D 5 inversion, the high In(2L)B 1 D 5 heterozygosity may be one reason for the preservation of parthenogenetic capability in Drosophila albomicans. It was reported that Drosophila mercatorum from natural populations which carried high heterozygosity are more capable of parthenogenesis than those from sexual inbreeding which carried high homozygosity (Templeton et al. [1976a]), so it may suggest that the high heterozygosity plays an important role in the maintenance of parthenogenesis. A Drosophila albomicans sexual strain #56.1 originating from Wulai population, which has the closest phylogenetic relationship to Japan population (Ohsako et al. ), was able to do parthenogenesis in spite of strong genetic drift and lacking selection for parthenogenesis with 40-year long-term sexual reproduction culture. The high In(2L)B 1 D 5 heterozygosity in #56.1 probably preserved parthenogenetic capability gene in the laboratory strain. In natural populations, high In(2L)B 1 D 5 heterozygosity was observed in winter in Wulai populations (Chang et al. ; Yang et al. ) and in the Japan population (Ohsako et al. ). Inversion heterosis may be responsible for the maintenance of parthenogenetic capability alleles in a population; however, inversion may not have direct relationship with the gene.
Parthenogenetic fecundity may be contributed by many loci widely distributed on whole genome. The parthenogenetic fecundity of F2 was shown as a quantitative trait, suggesting that there may have many loci with additive effect influencing fecundity. The significant correlation between the fecundity and the number of homozygous markers (ANOVA test, p < 0.001) suggests that the individuals with more PP markers may tend to have higher fecundity. That is, there are many loci with additive effect influencing parthenogenetic fecundity. The consistent significant correlation through jackknifing supports the quantitative trait model (i.e., many loci with small effect) and numerous alleles regulate the parthenogenetic fecundity. Identification of genes involved in parthenogenetic fecundity warrants our further investigation. In Drosophila mercatorum, the ‘coadapted genome’ for parthenogenesis was suggested through the decreasing fecundity with higher perturbation level, through recombinant lines generated by crossing different parthenogenetic strains (Templeton et al. [1976b]), and the coadaptation was formed effectively under the selection for parthenogenesis (Templeton ). Therefore, the additive effect for parthenogenetic fecundity is likely to respond to artificial selection for parthenogenesis (Ohsako and Fuyama ), and it might be coadapted with the gene for parthenogenetic capability.
In Drosophila albomicans, parthenogenetic capability is regulated by one genetic element located on 2L arm, and the high In(2L)B 1 D 5 heterozygosity may play a role in preserving parthenogenetic capability since the genetic element responsible for parthenogenetic capability is closely associated with In(2L)B 1 D 5 . Moreover, parthenogenetic fecundity shown as a quantitative trait by its continuous distribution is regulated by several additive loci, and through jackknifing, there is no highly influential one among the 14 markers. Therefore, we may infer that parthenogenetic fecundity is related to many genes in the genome including 2R, 2L, and neo-X chromosome arms.
We are grateful to Dr. Y. Fuyama for generously providing the parthenogenetic Drosophila albomicans strain, and we also thank the Sequencing Core Facility, Scientific Instrument Center at Academia Sinica for DNA sequencing. This work was partly funded by the grant (NSC100-2815-C002-142B) of National Science Council (Taiwan) for undergraduate student to CC.
- Annest JL, Templeton AR: Genetic recombination and clonal selection in Drosophila mercatorum . Genetics 1978, 89: 193–210.Google Scholar
- Carson HL: Selection for parthenogenesis in Drosophila mercatorum . Genetics 1967, 55: 157–171.Google Scholar
- Chang CH: Early-stage evolution of the neo-Y chromosomes in Drosophila albomicans. National Taiwan University, Taipei, Taiwan; 2011.Google Scholar
- Chang H, Chang SH, Lin FJ: Effects of climatic factors on the heterozygosity of In(2L)B 1 D 5 in Drosophila albomicans . B I Zool Acad Sinica 1987, 26: 39–45.Google Scholar
- Chang TP, Tsai TH, Chang H: Fusions of Muller's elements during chromosome evolution of Drosophila albomicans . Zool Stud 2008, 47: 574–584.Google Scholar
- Fuyama Y: Genetics of parthenogenesis in Drosophila melanogaster . II. Characterization of a gynogenetically reproducing strain. Genetics 1986, 114: 495–509.Google Scholar
- Hurst LD, Peck JR: Recent advances in understanding of the evolution and maintenance of sex. Trends Ecol Evol 1996, 11: 46–52. 10.1016/0169-5347(96)81041-XView ArticleGoogle Scholar
- Lewontin RC: The interaction of selection and linkage. I. General considerations; heterotic models. Genetics 1964, 49: 49–67.Google Scholar
- Markow TA: Parents without partners: Drosophila as a model for understanding the mechanisms and evolution of parthenogenesis. G3-Genes Genom Genet 2013, 3: 757–762.Google Scholar
- Matsuda M, Tobari YN: Genetic analyses of several Drosophila ananassae -complex species show a low-frequency major gene for parthenogenesis that maps to chromosome 2. Genet Res 2004, 83: 83–89. 10.1017/S0016672303006657View ArticleGoogle Scholar
- Maynard Smith J: The evolution of sex. Cambridge University Press, New York; 1978.Google Scholar
- Ohsako T, Fuyama Y: Drosophila albomicans , a new member of parthenogenetic Drosophila . Dros Inf Serv 1995, 76: 150.Google Scholar
- Ohsako T, Aotsuka T, Kitagawa O: The origins of the Japanese mainland population of Drosophila albomicans . Jpn J Genet 1994, 69: 183–194. 10.1266/jjg.69.183View ArticleGoogle Scholar
- Schwander T, Vuilleumier S, Dubman J, Crespi BJ: Positive feedback in the transition from sexual reproduction to parthenogenesis. Proc Biol Sci 2010, 277: 1435–1442. 10.1098/rspb.2009.2113View ArticleGoogle Scholar
- Simon JC, Delmotte F, Rispe C, Crease T: Phylogenetic relationships between parthenogens and their sexual relatives: the possible routes to parthenogenesis in animals. Biol J Linn Soc 2003, 79: 151–163. 10.1046/j.1095-8312.2003.00175.xView ArticleGoogle Scholar
- Stalker HD: Parthenogenesis in Drosophila . Genetics 1954, 39: 4–34.Google Scholar
- Suomalainen E: Parthenogenesis in animals. Adv Genet 1950, 3: 193–253. 10.1016/S0065-2660(08)60086-3View ArticleGoogle Scholar
- Templeton AR: The unit of selection in Drosophila mercatorum . II. Genetic revolution and the origin of coadapted genomes in parthenogenetic strains. Genetics 1979, 92: 1265–1282.Google Scholar
- Templeton AR: Nature and experimental parthenogenesis. In The genetics and biology of Drosophila. Academic, New York; 1983:343–398.Google Scholar
- Templeton AR, Carson HL, Sing CF: The population genetics of parthenogenetic strains of Drosophila mercatorium . II The capacity for parthenogenesis in a natural, bisexual population. Genetics 1976, 82: 527–542.Google Scholar
- Templeton AR, Sing CF, Brokaw B: The unit of selection in Drosophila mercatorum . I. The interaction of selection and meiosis in parthenogenetic strains. Genetics 1976, 82: 349–376.Google Scholar
- Yang YY, Lin FJ, Chang H: Comparison of recessive lethal accumulation in inversion-bearing and inversion-free chromosomes in Drosophila . Zool Stud 2002, 41: 271–282.Google Scholar
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