Table 1 Marker list with location, primer name, primer sequence, PCR annealing temperature, and restriction enzyme
From: Genetic analysis of parthenogenetic capability and fecundity in Drosophila albomicans
Location | Primer name | Primer sequence | Annealing temperature (°C) | Restriction enzyme | Reference |
|---|---|---|---|---|---|
Second chromosome | a28 | F: GGGGCACACTGATTTATTAAACAA | 57 | Alu I | Chang et al. ([2008]) |
R: TATTTTACGCCACAACTTGCAGCAC | |||||
a708 | F: GAAAAGGGCGAACAGATAGA | 55 | Xmn I | Chang ([2011]) | |
R: AACAGGAACATAGAAATCAC | |||||
A185D | F: CAAACGCTCTGGAATAATGG | 55 | Rsa I | Chang ([2011]) | |
R: CTCGGGAGTGTGGGGC | |||||
c4081 | F: GCCCTGGAACAAAGTGAAAA | 52 | Hae III | This study | |
R: AGTCTGCTGCGTATGGTCAA | |||||
c5237 | F: TATATGTTCCTCCTGATTGG | 55 | Pst I | Chang ([2011]) | |
R: AAGTTTAAACGCGAACTTTT | |||||
c5665 | F: TGAAACATTATTACCGCCTG | 60 | Hae III | This study | |
R: GATGACGACGACGATTCC | |||||
c7198 | F: GTGGGAAGCACGTTACAT | 57 | Mse I | Chang ([2011]) | |
R: CCATGAACAGTCTGAAGTTT | |||||
neo-X chromosome arm | a52 | F: TATTCATCGCATTCCACAT | 53 | Hae III | Chang et al. ([2008]) |
R: GGCTTCCTCAATCAACTG | |||||
a386 | F: GTTACGATTACGAAGAGTGC | 51 | Xmn I | Chang et al. ([2008]) | |
R: CTGCCGTGCTTATGTGAT | |||||
a1185 | F: ATTCTGTCGTTCGTTTTGA | 49 | Sty I | Chang et al. ([2008]) | |
R: GATTTCGGCTTACATTATTG | |||||
a1350 | F: TACGACCCCGTCAAAGGCTGTG | 52 | Hpa II | Chang ([2011]) | |
R: GGCTTGTATGCGATTCTGC | |||||
a1953 | F: GCCAACAGCGAGCCTTCT | 56 | Dde I | Chang et al. ([2008]) | |
R: GCGACCCAAGCACGAATC | |||||
c29 | F: CTGGGCAAAGAGTGTAGG | 57 | Rsa I | Chang et al. ([2008]) | |
R: CAGAAGGAGGGCGAAAA | |||||
c3242 | F: TTGAAGCGCAGTTTATGCAC | 62 | Ssp I | Chang ([2011]) | |
R: TACAACCACGACCTGGACAA |