DNA barcoding of Palaearctic Ulidiidae (Diptera: Tephritoidea): morphology, DNA evolution, and Markov codon models
© Galinskaya et al.; licensee Springer. 2014
Received: 14 April 2014
Accepted: 24 July 2014
Published: 20 August 2014
Here, for the first time, we report a barcoding survey of the dipterian family Ulidiidae (with two subfamilies Ulidiinae and Otitinae) coupled with morphology. To date, this is the first comprehensive analysis of the family that relies on molecular data. To reconstruct probable higher-level phylogenetic relationships between the genera of Ulidiidae, we exploited maximum likelihood and Bayesian inference, and additionally, we utilized a modern Markov model of codon substitutions for protein-coding genes coupled with the maximum likelihood approach to establish more realistic evolutionary scenarios connecting Ulidiinae and Otitinae.
Though we found morphological synapomorphic characters that clearly distinguish two groups of genera, formerly relating to two subfamilies, the monophyly of the clade Otitinae was not firmly supported by molecular phylogenetic reconstructions. The subfamily Ulidiinae was recovered as the basal likely paraphyletic group with high reliability. Overall, our results suggest the inclusion of the genera Homalocephala and Seioptera into the tribe Seiopterini (Otitinae). Three genera of Ulidiinae (Physiphora, Timia, Ulidia), eight genera of Otitinae (Ceroxys, Herina, Melieria, Myennis, Otites, Pseudotephritis, Seioptera, Tetanops), and the genus of Homalocephala with still unconfirmed systematic status were included in the analysis.
On all phylogenetic trees obtained in our survey, there is a clear tendency of clustering at the genus level with separation of (Physiphora + (Timia + Ulidia)) (Ulidiinae) and (Otitinae + Homalocephala). The genus Homalocephala takes basal or subbasal position relatively to Otitinae. Phylogenetic reconstruction based on Markov models of codon evolution provided a good resolution for our limited dataset.
KeywordsBarcoding Diptera DNA evolution Markov codon models Palaearctic Ulidiidae
Loew () was the first to propose a detailed classification of the family named Ortalidae (Figure 1). Within the family boundaries, he discerned seven groups: Pyrgotina, Ortalina, Cephalina, Platistoma, Pterocallina, Ulidiina, and Richardiina. Later, Ulidiinae, Pterocallinae, and Ortalinae have often been considered as the subfamilies in Muscaridae (Hendel [1910a], [b]) or as the separate families Ulidiidae, Pterocallidae (syn. Ulidiidae), and Ortalidae (syn. Otitidae) (Hendel ; Stackelberg ). Hennig (, , [1973a], [b]) discriminated Otitidae (including true Pterocallinae) and Ulidiidae (including Euxestinae) as independent families. Hennig (, ) showed that these two families might be only differentiated by the distiphallus structure and by the presence/absence of setulae on vein R1. Solid synapomorphic characters uniting Ulidiidae and Otitidae into a single monophyletic group were not found. In addition, Hennig (, ) included the genus Homalocephala Zetterstedt, 1838 into the family Ulidiidae. Nonetheless, Homalocephala was later moved to the family Otitidae (Hennig [1973a], [b]), whereas Homalocephala had been previously included either in Otitidae (Hendel [1910a], [b]) or in Pterocallidae (Stackelberg ). Most European dipterists (Richter [1970a], [b]; Soós ; Zaitzev ; Krivosheina and Zaitzev ; Greve ; Nartshuk ) use two separate family names, Ulidiidae and Otitidae, following Hennig’s earlier opinion (Hennig , , [1973a], [b]).
Based on American fly collections, Curran ([1934a], [b], 1944) proposed an alternative classification combining Ulidiinae with Otitinae into a single family called Otitidae. Besides Ulidiidae and Otitidae, he also included Pterocallinae, Richardiinae, and Platystomatinae in this family. At this time, the concept of synapomorphic and symplesiomorphic similarities was not yet developed; therefore, observed symplesiomorphic characters had been viewed as the evidence of systematic relationships. Thus, Ulidiinae + Otitinae + Pterocallinae + Richardiinae + Platystomatinae was thought to be a natural group, and the problem of acceptance of either five families or one family with five subfamilies had mostly classification agreement reasons. Steyskal (, , , , ) modified existing classification by the exclusion of Richardiidae and Platystomatidae, treating them as separate families, that was also supported by McAlpine (, ). Rohdendorf (, ), in his system of Diptera, considered the single family Otitidae as including the following four subfamilies: Otitinae, Ulidiinae, Euxestinae, and Pterocallinae. In addition, Evenhuis () determined a single subfamily Lipsaniinae for genus Lipsana Enderlein, 1940 (current syn. Physiphora of the family Otitidae) from the Australian region. Kameneva and Korneyev () showed that the genera Seioptera Kirby, 1817 and Pseudoseioptera Stackelberg, 1955 (Otitinae) are phylogenetically close to Homalocephala (Ulidiinae) and combined them into the tribe Seiopterini (Ulidiidae: Ulidiinae). The monophyly of the tribe is supported by several morphological characters: the absence of anepisternal bristles and the presence of two pairs of katepisternal bristles, the epandrium expanded in the anterocaudal direction, the distiphallus pubescent or short spinulose in its medial part, no sclerotized preglans or long bristles, and two pairs of spermathecae in females. Therefore, Kameneva and Korneyev (Kameneva and Korneyev, , ; Korneyev, ) included the rest of the Palaearctic Otitidae into the tribes Cephaliini, Otitini, and Myennidini, whereas the remaining Ulidiidae (without genus Homalocephala) were included into the tribes Ulidiini and Pterocallini, while considering all of the tribes within the scope of Ulidiidae. For uniting Ulidiinae and Otitinae into a single family, Kameneva and Korneyev used the following characters: non-developed fronto-orbital plates without strong setae (except in Chaetopsis Loew and close genera); absence of presutural supra-alar (except in Dyscrasis Hendel and some species of Otites Latreille) and katepimeral setae (presutural supra-alar present in the ground plan of most other families; katepimeral setae or setulae present in Platystomatidae, Ctenostylidae, Pyrgotidae, and Tephritidae); slightly bowed continuous Sc vein, neither bent anteriorly at right angle nor constricted before apex (bent anteriorly at right angle in Pyrgotidae Toxurini and most Tephritidae; constricted or broken before apex in Ctenostylidae, most Tephritidae, and some Pyrgotidae); R1 vein usually setulose only on apical portion (bare in all Ulidiini and most Lipsanini, and certain species of other tribes sometimes either bare or entirely setulose; bare in most or all Piophilidae, Pallopteridae, Lonchaeidae, and Richardiidae, and entirely setulose in Platystomatidae, Ctenostylidae, Pyrgotidae, and Tephritidae); a basal cubital (bcu) cell with triangular posterodistal lobe, except in Homalocephala Zetterstedt and few Pterocallini (absent in lower Tephritoidea, all Platystomatidae and Ctenostylidae, and in few genera of Pyrgotidae and Tephritidae); absence of male tergite 6 (rudimentary in few Lonchaeidae and Pallopteridae); bare male sternite 6 (setulose in lower Tephritoidea); absence of abdominal spiracles 6 and 7 in males (present in many lower Tephritoidea); phallus, when at rest, stored in pouch on ventral aspect of abdomen; apex of the phallus without separated glans (stored on dorsal side underneath tergite 6 with glans in other higher Tephritoidea); bare taeniae of eversible membrane without trichoid sensilla (with trichoid sensilla along whole length in most Piophilidae, Pallopteridae, and Lonchaeidae) and with two ducts of spermathecae; apically bifurcated single duct with two spermathecae, except in Lipsanini (two spermathecae on two ducts) (three spermathecal ducts in ground plan of Pyrgotidae and Tephritidae; two of them bifurcated close to vagina) (Kameneva and Korneyev ).
Since this is currently the most thorough and comprehensive taxonomic review of the group, we followed their hypothesis to consider the single family Ulidiidae with two subfamilies Ulidiinae and Otitinae.
Molecular phylogenetic studies of the superfamily Tephritoidea were conducted at the genus, subfamily, and family levels with the use of various molecular markers: 16S rDNA and COI (Zhang et al. ), COII (Smith and Bush ), 16S rDNA (Han et al. ), and 12S, 16S rDNA and COII (Han and Ro , ). Often phylogenetic inferences reached agreement with morphological data. Nevertheless, in most cases, a reconstruction of phylogenetic relationships among species/genera was based on relatively small sample surveys, which do not represent the full taxonomic diversity of the superfamily Tephritoidea. In addition, the phylogenetic schemes derived by different authors, with the implementation of various species samples and sets of molecular markers, make it difficult to compare these results. Importantly, the number of species from the family Ulidiidae included in the studies mentioned above was very small (two to three species, one individual).
In our study, we compared the external morphology and genital structures of Ulidiidae coupled with rigorous molecular phylogenetic analyses, in order to clarify its interspecific relationships. For species identification, we used external and genital morphology; for phylogenetic reconstruction, we performed stable morphological characters and sequence analysis based on a commonly used COI mitochondrial molecular marker. We found justification for the uniting of Ulidiinae and Otitinae into a single family.
Taxon sampling and morphological analysis
Sampling for subsequent phylogenetic analysis was focused on Ulidiidae flies of the Palaearctic region (Additional file 1). The specimens were identified, labeled, and stored in the fly collection of Zoological Museum at Lomonosov Moscow State University, Moscow, Russia (ZMUM).
For the investigation of external morphology and the anatomy of terminal male and female structures, in total, approximately 6,800 fly specimens belonging to 82 species of 3 genera of the subfamily Ulidiinae and 2,700 fly specimens from 107 species of 12 genera of the subfamily Otitinae were analyzed from museum collections. Morphological characters were examined and visualized under a stereomicroscope Stemi SV11 Apo Carl Zeiss (Oberkochen, Germany) and microscope Olympus CX41 (Shinjuku, Tokyo, Japan). The male and female terminalia were dissected out, treated with 10% KOH solution at approximately 80°C for several minutes, and observed in a droplet of glycerol under a compound light microscope. Photographic series were created with the use of CombineZM software (Hadley ).
This study is based mainly on the material deposited in the Zoological Institute of the Russian Academy of Sciences, Saint-Petersburg, Russia (ZISP), the Zoological Museum at Lomonosov Moscow State University, Moscow, Russia (ZMUM), the Hungarian Natural History Museum, Budapest, Hungary (HNHM), the Museum of Natural History at Humboldt University in Berlin, Germany (ZMHB), and Naturhistorisches Museum Wien, Austria (NHMW).
DNA analysis - extraction, amplification, and sequencing
The mitochondrial cytochrome c oxidase (COI) gene barcodes were successfully obtained for 69 specimens. Three genera of Ulidiinae (Physiphora (two species), Timia (four species), Ulidia (two species)), eight genera of Otitinae (Ceroxys (six species), Herina (two species), Melieria (four species), Myennis (one species), Otites (three species), Pseudotephritis (one species), Seioptera (one species), Tetanops (one species)), the genus Homalocephala (four species), and Psila fimetaria (L., 1761) (Psilidae) as an outgroup were included in a barcode analysis. Polymerase chain reaction (PCR) amplification and DNA sequencing were performed at the Canadian Centre for DNA Barcoding following standard throughput methods. The specimen information, vouchers, sample distribution, photographs. and GenBank accession numbers can be assessed through the Barcode of Life Data System (BOLD; http://www.barcodinglife.com) (Ratnasingham and Hebert ) in the publically available project ‘TGSPA-Schizophora of the Palaearctic.’ In order to broaden the sample space of the group Ulidiidae, we additionally obtained five COI barcodes of Physiphora clausa Macquart, 1843, Timia monticola Becker, 1906, Timia (Empyelocera) libani Gregor, 1970, Ulidia megacephala Loew, 1845, and Ulidia ruficeps Becker 1913 [GenBank:KC663633-KC663637]. DNA was extracted from whole specimens following the manufacturer’s protocol of the DIAtomTM DNA Prep 100 kit (Isogene, Russia). PCR amplification was performed using LCO1490 (forward, 5′-GGTCAACAAATCATAAAGATATTGG-3′) and HCO2198 (reverse, 5′-TAAACTTCAGGGTGACCAAAAAATCA-3′) primers (Folmer et al. ). The following are the conditions: PCR conditions - hot start 94°C for 5 min; pre-PCR 5 cycles - denaturation at 94°C for 1 min, annealing at 45°C for 1 min 30 s, elongation at 72°C for 1 min 30 s; and 35 cycles - denaturation at 94°C for 1 min, annealing at 50°C for 1 min 30 s, elongation at 72°C for 1 min. Amplification products were run on 1% TBE agarose gel (Sigma-Aldrich, St. Louis, MO, USA). PCR products were extracted from an agarose gel via the manufacturer’s protocol of the JETQUICK Gel Extraction Spin Kit 250 (GENOMED GmbH, Löhne, Germany). Sequencing was performed using a sequenator ABI PRISM 310 with reagents from an Applera kit (Norwalk, CT, USA). Contigs were made using CodonCode Aligner Ver126.96.36.199 (CodonCode Co.).
Sequence data analysis
The COI sequence alignment was produced using MAFFT v. 6.864b (Katoh et al. ) with the ‘auto’ option, which enables the user to automatically select an appropriate strategy among speed-oriented and accuracy-oriented methods. We included P. fimetaria (Psilidae) and four species of Drosophila Fallen, 1823 (derived from [GenBank:HQ979116.1, HQ979110.1, EU493633.1, JN019869.1]) as outgroups. They were selected based on current hypotheses of phylogeny: the superfamily Nothyboidea (including Psilidae) form a sister group to the superfamily Tephritoidea (including Ulidiidae) (McAlpine ; Gibson et al. ). Nothyboidea (Hennig , , [1973a], [b]) (=Diopsoidea (McAlpine ) = Tanypezoidea (Pape et al. )) includes families Tanypezidae, Strongylophthalmyiidae, Psilidae, Nothybidae, Megamerinidae, Syringogastridae, and Diopsidae, implying that the family Psilidae (superfamily Nothyboidea) has sister relationships with Tephritoidea. Belonging to the superfamily Ephydroidea, Drosophilidae represents a phylogenetically more progressive lineage relative to the basal Nothyboidea and Tephritoidea. Thus, Drosophilidae (superfamily Nothyboidea) is a sister group to Nothyboidea + Tephritoidea (Figure 2) (Rohdendorf ; McAlpine ; Hennig ; Wiegmann et al. ).
The substitution model of DNA sequence evolution was chosen among 88 competing models using a model test implemented in the IQ-Tree v. 0.9.4 software (http://www.cibiv.at/software/iqtree) (Minh et al. ) according to the model log-likelihood values. The TVM model, with a rate variation among sites following a gamma distribution (G), was selected according to the highest log-likelihood of −7706.4766. This model was used to construct a maximum likelihood (ML) tree. To do this, we used the IQ-Tree v. 0.9.4 software. In order to obtain branch support values, 1,000 bootstrap replicates were performed in the same software utilizing the chosen evolutionary model. In addition, a replicated Bayesian analysis coupled with Markov chain Monte Carlo (MCMC) simulations starting from an initial random tree was performed in MrBayes v 3.2.1. (http://mrbayes.sourceforge.net/) (Ronquist and Huelsenbeck ) for four million generations in two independent runs with a sampling frequency every 500 generations. All trees that were sampled before the two runs reached a value of standard deviation less than 0.015 were discarded as burn-in. The resulting trees were summarized to a consensus, and posterior probabilities (PP) as branch statistical support were then calculated. Since the ML and Bayesian trees agreed on one topology, we drew the bootstrap support values (in %) from 1,000 bootstrap trees onto the Bayesian tree using the IQ-Tree software, thus combining bootstrap support (BS) with posterior probability values.
In addition to a typical phylogenetic analysis based on common models of nucleotide substitutions in DNA sequence, we reconstructed phylogenetic relationships utilizing a model of codon evolution. Markov models of codon substitution have emerged as a powerful method modeling biologically more realistic scenarios for protein-coding sequences (Gil et al. ). The most important advantages of codon models over DNA models are being achieved by naturally incorporating selection regimes acting on protein and the structure of the genetic code as relevant model parameters. For further phylogenetic inference, the YAP parametric codon model was a priori selected, reasoning that estimation of the non-synonymous to synonymous substitution ratio (ω) is not affected by base composition at each codon compared to other codon models, namely GY and MG (for details, see Yap et al. ()). The selective variability (distribution of ω ratios) among codon sites was modeled using the model M3 with three discrete site classes (Yang et al. ). Empirical codon frequencies were estimated under CFx4 option, which enables correction for absence of stop codons (Kosakovsky Pond et al. ). Then, ten initial trees generated by the BioNJ method corresponding to ten random starts were estimated. Optimization of each tree topology was carried out using simultaneously two ML heuristic tree search algorithms - nearest neighbor interchange (NNI) and subtree pruning and regrafting (SPR). The most likely tree was obtained with SPR moves (log-likelihood = −6781.1666). The branch support was assessed by the Bayesian-like modification of the approximate likelihood ratio test (aBayes) (Anisimova et al. ). aBayes was defined to be the most powerful among other branch support methods based on an approximate likelihood ratio test. Although aBayes supports tend to be very similar to PP supports, the aBayes method exhibits more conservative behavior and robustness to model violations (Anisimova et al. ). All of the above procedures and codon ML tree construction were implemented in CodonPhyML Ver1.0 (http://sourceforge.net/projects/codonphyml/) (Gil et al. ).
For the barcoding data, the distance matrix was produced under the Kimura two-parameter (K2P) (Kimura ) model of base substitution with uniform rates using MEGA 5 (Tamura et al. ). In addition, we calculated uncorrected p-distances as an alternative distance metric that may be preferable to K2P for short, closely related sequences. The intraspecific variation and interspecific divergence levels were investigated by comparing pairwise K2P distances within and between fly species.
Sequence details and nucleotide composition
The alignment of 78 COI sequences of unequal lengths (min = 246 bp, max = 701 bp), including 4 P. fimetaria and 4 Drosophila outgroup sequences, contained 704 positions without gaps; 269 sites were variable, 251 of which were identified as parsimony-informative. Although short COI sequences (<600 bp) may not provide sufficient information for phylogenetic reconstruction, short barcode sequences (<150 bp, mini-barcodes) have been reported to provide an efficient taxonomic signal for species discrimination (Meusnier et al. ). In their survey, a correct species identification rate of 95% was achieved with 250-bp barcodes. Estimated average base frequencies for the barcode region (all sites) in our dataset were A 28.53%, C 19.84%, G 17.69%, T 33.94% (χ 2 = 38.82, df = 3, P = 1.896e − 08; Additional file 3). As expected, the A/T bias (79.35%) was significant for the third codon positions of the sequence (χ 2 = 223.03, df = 1, P < 2.2e − 16) and considerably greater than at first (50.05%) and second (58.03%) positions (χ 2 = 51.69, df = 2, P = 5.972e − 12).
We examined external morphology of approximately 6,800 fly specimens belonging to 82 species of 3 genera in the subfamily Ulidiinae and 2,700 fly specimens from 107 species of 12 genera in the subfamily Otitinae. Based on this analysis, we confirmed morphological characters that are able to separate all studied genera. In particular, the genital structures (surstyles, phallus, and phallus apodeme) were shown to be the most useful for distinguishing species. We moved the results of the morphological analysis to the ‘Discussion’ section for easier perception.
Our phylogenetic analysis suggests the existence of three major clusters - Physiphora (Ulidiinae), Timia + Ulidia (Ulidiinae), and Otitinae + Homalocephala (Figures 4 and 5B). Inclusion of Homalocephala into Otitinae coincides with Hennig’s classification , whom previously considered it within Ulidiidae. The subfamily Ulidiinae (Physiphora + (Timia + Ulidia)) forms a basal paraphyletic group (BS = 33, PP = 0.64, aBayes = 0.60) nested between P. fimetaria and Otitinae. Nevertheless, morphologically these genera are similar in having a bare vein R1, as well as in genital structures (non-rupted closed hypandrium, well-developed parameres, a bare distiphallus (rarely with a group of setae in the center of distifallus) with membranous lobes, and a phallus apodeme with lateral lobes). Thus, there might be symplesiomorphic similarity. The subfamily Otitinae occupies the apical position on all of the phylogenetic trees (Figures 5 and 6). Despite this, the lineage was not statistically recovered as a monophyletic group, with low support values (BS = 8, PP = 0.45, aBayes = 0.58). Morphologically, the monophyly of Otitinae (without Homalocephala) was supported by a spinulose phallus, absence of the glans, absence of membranous lobes on the distiphallus and by the presence of the female sternal apodemes. We assume these characters to be an apomorphic similarity. Interestingly, the Homalocephala genus takes intermediate position between Ulidiinae Otitinae, which can be corroborated by previous studies (including Hennig [1973a], [b]). Recovery of basal and subbasal positions of the Seioptera species within Otitinae is partially supporting the hypothesis of Kameneva and Korneyev (), which suggests inclusion of the Homalocephala and Seioptera genera into the Seiopterini tribe, however, within Ulidiinae. Our results confirm the uniting of Ulidiinae and Otitinae into a single family.
Ulidia (Palearctic genus, 21 species) and Timia (Palearctic genus, 59 species) most likely form a monophyletic (BS = 34, PP = 0.90, aBayes = 0.91) cluster, although these two genera do not create particular clusters within it. The morphological data that closely relates these two genera, the overall external characters and genital structures and having little or no hiatus, firmly supports the phylogenetic inference. Group specialists often encounter issues in separation between Ulidia and Timia, so the possible merging of these genera has been suggested. The differences used for separating Ulidia and Timia so far are mainly the following: the frons is smooth (in Timia) versus dimpled (in Ulidia) (with some exceptions); the head and thorax are microtrichose (in Timia) versus bare (in Ulidia, but some assigned to Timia have a shiny head and thorax, and the frons of Ulidia metope Kameneva,  is widely microtrichose) (Chen and Kameneva ; Kameneva ). The monophyletic status of the group Timia + Ulidia is also confirmed by the distribution of the species with a shared ecological niche - the majority of Ulidia and Timia occur in arid and semiarid areas of the Palaearctic. Based on these data, it is more likely that the formal characters defined Ulidia and Timia, unambiguously identifying just a fraction of species in each genus. Likewise, the subgenera Timia and Empyelocera in the genus Timia seem to be unnatural taxa, which is only differentiated by width of parafacial area (Zaitzev ). This assumption is congruent with our hypothesis that the genera Ulidia and Timia have recently diverged and are currently on a course of differentiation. Within these groups, typical representatives as well as transitional forms can be found. The molecular phylogenetic reconstruction does not exclude a more complicated scenario such as Ulidia and Timia not being natural groups but forming a cluster of several subgroups connected with multiple transitions. Timia have transparent wings, some species have dark basal costal (bc), costal (c), and subcostal (sc) cells and with apical wing spot. A basal bcu cell has a well-developed posteroapical projection, whose length has an identification value (Figure 7Z,A1,A2,A3). Male surstyles do not possess strong prensisetae, occasionally with setae on the inner lobe (Figure 8Y,Z,A1,A2). The genus Ulidia possesses a poorly developed wing pattern, the species Ulidia megacephala, Ulidia melampodia Loew, 1873, Ulidia salonikiensis Hennig, , Ulidia kandybinae Zaitzev, , Ulidia splendida Zaitzev, , Ulidia nigricubitalis Zaitzev, , and Ulidia transcaspica Galinskaya, 2011 exhibit the greatest development of the character: dark costal, subcostal, and (sometimes) bcu cells and an apical wing spot (Figure 7A4,A5). Ulidia possess three smooth spermathecae with two of them having a collective sperm duct. Male genitalia has the shape of the phallus tip (glans) in combination with a bare distiphallus (rarely with a group of setae in the center of the distiphallus) and distiphallus with membranous lobes. Male surstyles do not possess strong prensisetae, occasionally with setae on the inner lobe (Figure 8A3,A4). It is important to note that within Timia, we found three species (Timia albifacies Gorodkov and Zaitzev, 1986, Timia gussakovskyi Gorodkov and Zaitzev, 1986, and Timia komarowi Mik, 1889) that have the distiphallus in the middle part with an area densely covered by relatively strong setae. We hypothesize that setae on the distiphallus of Timia and Otitinae emerged in parallel, possibly having unique origins.
Homalocephala species (nine species total: four Holarctic, four Palaearctic, and one Nearctic) have always been included into the clade Otitinae with different, lowly supported clustering patterns on the Bayesian/ML tree (Figure 5A) and codon ML tree (Figure 6A). On the codon ML tree, the Homalocephala cluster likely exhibits sister relationships (aBayes = 0.84) with the Otites + Tetanops + Herina + Ceroxys + Melieria group (aBayes = 0.85). Homalocephala species possess the following relevant morphological characters: no glans on the distiphallus, two katepisternal setae, and four spherical spermathecae. This relates these species to the Seioptera and Pseudoseioptera genera, whereas having vein R1 covered with setulae along its full length relates Homalocephala to Pseudoseioptera. We notice that Homalocephala and Seioptera genera were placed basally or subbasally relatively to Otitinae (Figures 5A and 6A). Species of Homalocephala possess transparent wings, occasionally with apical spots (on apexes of veins R2 + 3 and R4 + 5) or with medial spots occupying pterostigma and sometimes extending downward. The bcu cell is enclosed with arcuate vein without breaking (Figure 7T,U,V,W). Male surstyles possess figured prensisetae (Figure 8S,T,U,V) that are typical only of genera Homalocephala, Pseudoseioptera, and Ceroxys and are probably the result of parallel origin or convergent evolution. Interestingly, the areal of Homalocephala partially coincide with the areal of Pseudoseioptera inhabiting Holarctic temperate forests; moreover, both of their larvae live under the bark of falling trees. The position of this genus is disputed, with differerent scientists including it in Otitidae, Ulidiidae, or Pterocallidae (Kameneva and Korneyev ; Hendel [1910a], [b], ; Stackelberg ; Hennig , ; Richter [1970a], [b]; Soós ; Zaitzev ; Krivosheina and Zaitzev ; Greve ; Nartshuk ; Curran [1934a], [b]; Steyskal , ; McAlpine , ; Rohdendorf ; Evenhuis ; Frey ) (Figure 1). Based on phylogenetic inferences about the genus, Homalocephala (Figures 5A and 6A) tend to be placed within the Otitinae clade, though the taxonomic status of the genus needs to be extensively revised. Morphologically, the Homolocephala lineage remains unresolved too, as most of the characters examined are plesiomorphic and cannot reliably relate it to either Otitinae or Ulidiinae.
Melieria contains 38 species: 2 Holarctic, 4 Nearctic, and 29 Palaearctic. In our study, Melieria omissa (Meigen, 1826), Melieria cana, Melieria acuticornis (Loew, 1854), and Melieria crassispennis (Fabricius, 1794) exhibited a tendency to form a single cluster with an apical topology (Figures 5A and 6A). While the codon ML tree gave the genus high branch support (aBayes = 0.98), the Bayesian/ML tree ambiguously clustered it with Ceroxys munda (BS = 10, PP = 0.51). Interestingly, Melieria shows maximal levels of intraspecific diversity, whereas interspecific comparisons within the genus attain the minimal distances. Perhaps, these results suggest deep conspecific diversity of M. cana, which in turn may indicate a current process of speciation. Species of Melieria possess an R1 vein with setulae in its apical portion and a bcu cell with very short posteroapical extension (Figure 7H,I,J,K). The male distiphallus is covered by long bristles, lacks membranous lobes, and lacks a glans; it also has surstyles with two to five prensisetae (Figure 8G,H,I,J).
The single representative of Tetanops Fallén, 1820 (Holarctic genus with eight Nearctic and six Palaearctic species) - Tetanops sintenisi Becker, 1909 - was placed with Otites formosa on the Bayesian/ML tree with weak support (BS = 15, PP = 0.69), but on the codon ML tree, it was placed within the lowly supported clade containing Herina lugubris, Ceroxys hortulana, and Otites lamed (aBayes = 0.77). T. sintenisi possesses an R1 vein with setulae and a bcu cell with short posteroapical extension (Figure 7S). The male distiphallus is covered by long strong bristles and lacks membranous lobes and a glans; it has surstyles with two to eight prensisetae on the medial lobe (Figure 8R).
Herina R.-D., 1830 has 31 species: 19 Palaearctic species, 4 Nearctic, 6 Oriental, and 2 Neotropic. Herina lugubris (Meigen, 1826) and Herina frondescentiae (L., 1758) were differently clustered with low branch support values (Figures 4A and 5A). Species of Herina possess an R1 vein with setulae and a bcu cell with short, often indistinct posteroapical extension (Figure 7F,G). The male distiphallus is covered by short strong bristles and lacks membranous lobes and a glans; it has surstyles with prensisetae and its lobes are often elongated and modified (Figure 8E,F). Herina are generally widespread in the majority of zoogeographic regions, excluding the Afrotropic (Kameneva and Korneyev ).
Representatives of Otites Latr., 1804 (31 species: 5 Nearctic and 26 Palaearctic species) - Otites guttata (Meigen, 1830), Otites lamed (Schrank, 1781), and Otites formosa were scattered across all the trees showing no particular clustering patterns (Figures 5A and 6A). Species of Otites possess an R1 vein with setulae and a bcu cell with short posteroapical extension (Figure 7N,O,P). The male distiphallus is covered by strong bristles and lacks membranous lobes and a glans. The medial lobes of the surstyles are covered by an abundance of prensisetae (15 to 40), which clearly represent the genera’s character (Figure 8M,N,O). Otites is the only Holarctic genus, with the majority of the other species distributed in Southern Europe.
The single representative of Seioptera (two species: one Holarctic and one Nearctic) - S. vibrans (Linnaeus, 1758) - was nested within the lineage Myennis on the codon ML tree (aBayes = 0.72; Figure 6A). However, on the Bayesian/ML tree, it showed different topology clustering with Myennis sibirica (BS = 28, PP = 0.57; Figure 5A). Morphologically, this genus is close to Pseudoseioptera and Homalocephala based on the following characters: no glans on the distiphallus, two katepisternal setae, and four spherical spermathecae. Species of Seioptera possess a bare R1 vein, a cup cell with short posteroapical extension, and an sc cell darkened and an apical spot (Figure 7R). The male distiphallus lacks a glans and membranous lobes and is covered by relatively strong bristles along its full length; it has surstyles with two big prensisetae on the medial lobe (Figure 8Q).
Ceroxys (Macquart, 1835) contains 16 species: 15 Holarctic and 1 Nearctic. Ceroxys hortulana (Rossi, 1790), Ceroxys urticae (L., 1758), Ceroxys morosa (Loew, 1873), Ceroxys cinifera (Loew, 1846), and Ceroxys munda (Loew, 1868) exhibit highly inconsistent clustering patterns on all of the trees (Figures 5A and 6A). Only C. urticae and C. morosa jointly cluster with moderate branch support (BS = 18, PP = 0.81, aBayes = 0.99). Species of Ceroxys possess an R1 vein with setulae and a bcu cell with short posteroapical extension (Figure 7A,B,C,D,E). The male distiphallus is covered with strong bristles and lacks membranous lobes and a glans; it has surstyles with prensisetae (Figure 8A,B,C,D).
Representatives of Myennis R.-D., 1830 (four Palaearctic species) - Myennis octopunctata (Coquebert, 1798) and Myennis sibirica Portschinsky, 1892 - formed the single cluster with non-significant branch support on the codon ML tree (aBayes = 0.72; Figure 6A). No particular clustering was observed on the Bayesian/ML tree. Species of Myennis possess an R1 vein with setulae and a bcu cell with short posteroapical extension (Figure 7L,M). The male distiphallus is covered by short strong bristles and lacks membranous lobes and a glans; the lateral lobes of the surstyles have two to three prensisetae (Figure 8K,L). The genus Myennis is mostly restricted to forest zones. Myennis and Seioptera genera form a singe branch on all the phylogenetic hypotheses.
The single representative of Pseudotephritis Johnson, 1802 (five species: two Palaearctic and three Nearctic) - Pseudotephritis corticalis (Loew, 1873) - is not consistently placed on all of the trees. Species of Pseudotephritis possess an R1 vein with setulae and a bcu cell with long posteroapical extension (Figure 7Q). The male distiphallus is covered by strong bristles and lacks membranous lobes and a glans; it has surstyles with prensisetae (Figure 8P).
In summary, based on phylogenetic inference, the subfamily Otitinae did not form a statistically supported monophyletic group. However, we found morphological synapomorphic characters that may unambiguously distinguish Otitinae from Ulidiinae, namely a spinulose phallus, the absence of the glans, the absence of membranous lobes on the distiphallus, and the presence of the female sternal apodemes. Presumably, the COI region has no sufficient phylogenetic signal to resolve genera divergence.
The phylogenetic analysis of COI showed satisfactory results in recovering some major lineages of the Ulidiidae family. On all phylogenetic trees obtained in our survey, there is a clear tendency of clustering at higher levels with separation of (Physiphora + (Timia + Ulidia)) (Ulidiinae) and (Otitinae + Homalocephala). Otitinae take apical position within the Ulidiidae family. However, monophyly of the Ulidiinae subfamily was not supported, forming basal paraphyletic group. Our tree inferences provide evidence that the Homalocephala genus has ambiguous evolutionary relationships with other Ulidiinae and Otitinae that was also shown by morphological analyses. In addition, our results support the inclusion of the Homalocephala and Seioptera genera into the Seiopterini tribe that was initially proposed by Kameneva and Korneyev ().
Additional material from the subfamilies Ulidiinae and Otitinae would aid to further investigation to confirm the phylogenetic status of Ulidiinae, Otitinae, and the genus Homalocephala.
We also conclude that analysis of other molecular markers may help to support the hypothesis of an evolutionary split having occurred between Ulidiinae and Otitinae. Nevertheless, phylogenetic reconstruction based on Markov models of codon evolution provides a good resolution for our limited dataset. Most likely, the codon models coupled with the ML approach are capable of capturing more general phylogenetic patterns, making them very promising and attractive tools for modern systematic entomology.
We thank Ilya A. Zakharov and Irina I. Goryacheva for their assistance with the genetic analysis and valuable comments on early stages. We are also grateful to Nicola Palmieri and Carolin Kosiol for their help with the data mining and suggestions on the implementation of codon models. We are deeply indebted to Joachim Ziegler (MNHB), Peter Sehnal (NHMW), Laslo Papp (HNHM), Olga G. Ovchinnikova (ZISP), and Andrey L. Ozerov (ZMMU) for the loan of material. We are also grateful to Elena V. Dmitrieva for her assistance with the microscopes. We thank Alex Borisenko for his help with the submission of our specimens to barcoding and technical support. We additionally thank Andy Fogel for his valuable comments and constructive criticism. Preparation of the barcodes and specimen documentation were performed at the Canadian Centre for DNA Barcoding, University of Guelph and funded by the Government of Canada through Genome Canada and the Ontario Genomics Institute (2008-OGI-ICI-03). The work of Tatiana V. Galinskaya was partially supported by RFBR (research projects no. 13-04-01638 and no. 14-04-31932_mol_a). We are also indebted to the anonymous reviewers for providing insightful comments and constrictive criticism.
- Andersson H: Revision of Swedish Homalocephala Zetterstedt (Diptera, Otitidae). Entomologisk Tidskrift 1991,112(1–2):27–32.Google Scholar
- Anisimova M, Gil M, Dufayard JF, Dessimoz C, Gascuel O: Survey of branch support methods demonstrates accuracy, power, and robustness of fast likelihood-based approximation schemes. Syst Biol 2011, 60: 685–699. 10.1093/sysbio/syr041View ArticleGoogle Scholar
- Chen XL, Kameneva EP: A review of Ulidia Meigen (Diptera: Ulidiidae) from China. Zootaxa 2009, 2175: 42–50.Google Scholar
- Curran CH: The Diptera of Kartabo, Bartica District, British Guiana. Bull Am Mus Nat Hist 1934a,66(3):287–533.Google Scholar
- Curran CH: The families and genera of North American Diptera. Ballou Press, New York; 1934b.View ArticleGoogle Scholar
- Evenhuis NL: Family Otitidae. In Catalog of the Diptera of Australasian and Oceanian Regions. Edited by: Evenhuis NL. Bishop Museum Press, Honolulu; 1989:479–481. 10.5962/bhl.title.49897View ArticleGoogle Scholar
- Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R: DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotechnol 1994, 3: 294–299.Google Scholar
- Frey R: Beitrag zur Kenntnis der ostasiatischen Plastystomiden (Diptera). Notulae Entomologicae 1964, 44: 1–19.Google Scholar
- Gibson JF, Skevington JH, Kelso S: Placement of Conopidae (Diptera) within Schizophora based on mtDNA and nrDNA gene regions. Mol Phylogenet Evol 2010,56(1):91–103. 10.1016/j.ympev.2010.03.026View ArticleGoogle Scholar
- Gil M, Zanetti MS, Zoller S, Anisimova M: CodonPhyML: fast maximum likelihood phylogeny estimation under codon substitution models. Mol Biol Evol 2013,30(6):1270–1280. 10.1093/molbev/mst034View ArticleGoogle Scholar
- Greve L: 3.12. Family Otitidae. In Contributions to a Manual of Palaearctic Diptera. Edited by: Papp L, Darvas B. Science Herald, Budapest; 1998:187–192.Google Scholar
- Hadley A: CombineZM. Open source image processing software package for creating extended depth of field images. 2007.Google Scholar
- Han H-Y, Ro K-E: Molecular phylogeny of the superfamily Tephritoidea (Insecta: Diptera): new evidence from the mitochondrial 12S, 16S and COII genes. Mol Phylogeny Evol 2005,34(2):416–430. 10.1016/j.ympev.2004.10.017View ArticleGoogle Scholar
- Han H-Y, Ro K-E: Molecular phylogeny of the family Tephritidae (Insecta: Diptera): new insight from combined analysis of the mitochondrial 12S, 16S, and COII genes. Mol Cells 2009, 27: 55–66. 10.1007/s10059-009-0005-3View ArticleGoogle Scholar
- Han H-Y, Ro K-E, McPheron BA: Molecular phylogeny of the subfamily Tephritinae (Diptera: Tephritidae) based on mitochondrial 16S rDNA sequences. Mol Cells 2006, 1: 78–88.Google Scholar
- Hendel F: Uber acalyptrate Musciden. Wiener Entomologische Zeitung 1910a,29(2):123–127.Google Scholar
- Hendel F: Diptera, Fam. Muscaridae, Subfam. Ulidiinae. In Genera insectorum. Edited by: Wytsman P. Wytsman Verl, Bruxelles; 1910b:1–76.Google Scholar
- Hendel FG: Zweiflügler oder Diptera. II. Allgemeiner Teil. Die Tierwelt Deutschlands 11, Jena. 1928.Google Scholar
- Hennig W: 46. Otitidae. In Die Fliegen der Palaearktischen Region. Edited by: Lindner E. E. Schweizerbart, Stuttgart; 1939:1–79.Google Scholar
- Hennig W: 45. Ulidiidae. In Die Fliegen der Palaearktischen Region. Edited by: Lindner E. E. Schweizerbart, Stuttgart; 1940:1–34.Google Scholar
- Hennig W: Die Familien der Diptera Schizophora und ihre phylogenetischen Verwandschaftsbeziehungen. Beitr Entomol 1958, 8: 505–688.Google Scholar
- Hennig W: Neue Untersuchungen uber die Familien der Diptera Schizophora (Diptera: Cyclorrhapha). Can Entomologist 1971, 101: 589–633. 10.4039/Ent101589-6View ArticleGoogle Scholar
- Hennig W (1973a) Ordnung Diptera (Zweiflügler). In: De Gruyter W (ed) Handbuch der Zoologie, vol 31. Berlin, pp 1–337
- Hennig W: Ordnung Diptera (Zweiflügler). In Handbuch der Zoologie. Edited by: Helmcke JG. Lfg, Berlin; 1973b:1–337.Google Scholar
- Kameneva EP: New and little-known Ulidiidae (Diptera, Tephritoidea) from Europe. Vestn Zool 2008,42(5):427–454.Google Scholar
- Kameneva EP: A peculiar new species of the Genus Ulidia (Diptera, Ulidiidae) from Middle Asia. Vestn Zool 2010,44(2):177–181.Google Scholar
- Kameneva EP, Korneyev VA (1994) Holarctic genus Pseudoseioptera Stackelberg (Diptera: Ulidiidae (=Otitidae)). Part I. Phylogenetic relationships and taxonomic position. J Ukr Entomol Soc 1(2):65–72
- Kameneva EP, Korneyev VA: Myennidini, a New Tribe of the Subfamily Otitinae (Diptera: Ulidiidae), with Discussion of the Suprageneric Classification of the Family. In Israel Journal of Entomology Biotaxonomy of Tephritoidea. Edited by: Freidberg A. Proceedings of the Tephritidological Meeting, Israel; 2006:497–586.Google Scholar
- Katoh K, Misawa K, Kuma K, Miyata T: MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Res 2002,30(14):3059–3066. 10.1093/nar/gkf436View ArticleGoogle Scholar
- Kimura M: A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980, 16: 111–120. 10.1007/BF01731581View ArticleGoogle Scholar
- Korneyev VA: Phylogenetic Relationships among the Families of the Superfamily Tephritoidea. In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. Edited by: Aluja M, Norrbom AL. CRC Press, Boca Raton; 1999:3–22. 10.1201/9781420074468.sec1View ArticleGoogle Scholar
- Kosakovsky Pond S, Delport W, Muse SV, Scheffler K: Correcting the bias of empirical frequency parameter estimators in codon models. PLoS One 2010,5(7):e11230. 10.1371/journal.pone.0011230View ArticleGoogle Scholar
- Krivosheina NP, Zaitzev AI: Phylogenetic and evolutionary ecology of dipterous insects. Results in science and technology. Entomol Ser 1989, 9: 1–162. [in Russian]Google Scholar
- Loew H: Die europaischen Ortaliden. Z Gesamte Naturwissenschaften Halle 1868, 32: 1–11.Google Scholar
- McAlpine JF: A revised classification of the Piophilidae, including “Neottiophilidae” and “Thyreophoridae” (Diptera: Schizophora). Mem Entomol Soc Can 1977, 103: 1–66. 10.4039/entm109103fvView ArticleGoogle Scholar
- McAlpine JF: Morphology and Terminology – Adults. In Manual of Nearctic Diptera (Monograph 27). Edited by: McAlpine JF, Peterson BV, Shewell CE, Teskey HJ, Vockeroth JR, Wood DM. Research Branch, Agriculture Canada, Ottawa; 1981:9–63.Google Scholar
- McAlpine JF: Phylogeny and Classification of the Muscomorpha. In Manual of Nearctic Diptera (Monograph 32). Edited by: McAlpine JF, Peterson BV, Shewell CE, Teskey HJ, Vockeroth JR, Wood DM. Research Branch, Agriculture Canada, Ottawa; 1989:1397–1518.Google Scholar
- Meusnier I, Singer GAC, Landrym J-F, Hickey DA, Hebert PDN, Hajibabaei M: A universal DNA mini-barcode for biodiversity analysis. BMC Genomics 2008, 9: 214.9. 10.1186/1471-2164-9-214View ArticleGoogle Scholar
- Minh BQ, Nguyen MAT, von Haeseler A: Ultrafast approximation for phylogenetic bootstrap. Mol Biol Evol 2013, 30: 1188–1195. 10.1093/molbev/mst024View ArticleGoogle Scholar
- Nartshuk EP: Key to families of Diptera (Insecta) of the fauna of Russian and adjacent countries. In Proceedings of the Zoological Institute. Edited by: Zaitzev VF. Russia, Leningrad; 2003:1–251. [in Russian]Google Scholar
- Ong PS, Luczon AU, Quilang JP, Sumaya AMT, Iban EJC, Salvador DJ, Fontanilla IKC: DNA barcodes of Philippine accipitrids. Mol Ecol Resour 2011, 11: 245–254. 10.1111/j.1755-0998.2010.02928.xView ArticleGoogle Scholar
- Pape T, Blagoderov V, Mostovski MB: Order Diptera Linnaeus, 1758. In Animal Biodiversity: An Outline of Higher-Level Classification and Survey of Taxonomic Richness. Edited by: Zhang ZQ. Zootaxa, Magnolia Press, New Zealand; 2011:222–229.Google Scholar
- Ratnasingham S, Hebert PDN: The Barcode of Life Data System. Mol Ecol Notes 2007, 7: 355–364. [www.barcodinglife.org] 10.1111/j.1471-8286.2007.01678.xView ArticleGoogle Scholar
- Richter V: 60. Family Otitidae. In Key to Species of European Territory of the USSR in Five Volumes. Edited by: Bey-Bienko GY. Nauka, Leningrad; 1970a:123–130. [in Russian]Google Scholar
- Richter VA: 61. Family Ulidiidae. In Key to species of European territory of the USSR in five volumes. Edited by: Bey-Bienko GY. Nauka, Leningrad; 1970b:130–132. [in Russian]Google Scholar
- Rohdendorf BB: The Historical Development of Diptera. University of Alberta Press, Edmonton; 1974.Google Scholar
- Rohdendorf BB: System and Phylogeny of Diptera. In Systematics and Evolution of Diptera. Edited by: Gorodkov KB. ZIN AN USSR, Leningrad; 1977:81–88.Google Scholar
- Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 2003, 19: 1572–1574. 10.1093/bioinformatics/btg180View ArticleGoogle Scholar
- Smith JJ, Bush GL (1997) Phylogeny of the genus Rhagoletis (Diptera: Tephritidae) inferred from DNA sequences of mitochondrial cytochrome oxidase II. Mol Phylogenet Evol 7(1):33–43
- Soós Á: Family Otitidae (Ortalidae). In Catalogue of Palaearctic Diptera. Edited by: Soós A, Papp L. Akadémiai Kiádo. Budapest and Elsevier Science Publishers, Amsterdam; 1984:45–59.Google Scholar
- Srivathsan A, Meier R: On the inappropriate use of Kimura-2-parameter (K2P) divergences in the DNA-barcoding literature. Cladistics 2012, 28: 190–194. 10.1111/j.1096-0031.2011.00370.xView ArticleGoogle Scholar
- Stackelberg AA: Key to Diptera of European part of USSR. AN USSR, Leningrad; 1933.Google Scholar
- Steyskal GC: The genera of Platystomatidae and Otitidae known to occur in America north of Mexico (Diptera, Acalyptratae). Ann Entomol Soc Am 1961,54(3):401–410.View ArticleGoogle Scholar
- Steyskal GC: Family Otitidae. In A Catalog of the Diptera of America North of Mexico. Edited by: Stone A, Sabrosky CW, Wirth WW, Foote RH, Coulson JR. United States Department of Agriculture, Washington; 1965:642–654.Google Scholar
- Steyskal GC: 54. Family Otitidae (Ortalidae; including Pterocallidae, Ulidiidae). In A Catalogue of the Diptera of the Americas South of the United States. Edited by: Vanzolini PE, Papavero N. Dept. Zool. Secretaria Agric, São Paulo; 1968:1–31.Google Scholar
- Steyskal GC: Family Otitidae. In A Catalog of the Diptera of the Oriental Region. Edited by: Delfinado MD, Hardy DE. The University Press of Hawaii, Honolulu; 1977:165–167.Google Scholar
- Steyskal GC: Otitidae. In Manual of Nearctic Diptera (Monograph 28). Edited by: McAlpine JF, Peterson BV, Shewell CE, Teskey HJ, Vockeroth JR, Wood DM. Research Branch, Agriculture Canada, Ottawa; 1987:799–808.Google Scholar
- Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S: MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011,28(10):2731–2739. 10.1093/molbev/msr121View ArticleGoogle Scholar
- White IM, Headrick DM, Norrbom AL, Carroll LE: Glossary. In Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. Edited by: Aluja M, Norrbom AL. CRC Press, Boca Raton; 1999:881–924. 10.1201/9781420074468.sec8View ArticleGoogle Scholar
- Wiegmann BM, Trautwein MD, Winkler I, Barr NW, Kim J-W, Lambkin C, Bertone MA, Cassel BK, Bayless KM, Heimberg AM, Wheeler BM, Skevington JH, Pape T, Sinclair BJ, Borkent A, Blagoderov V, Caravas J, Kutty SN, Peterson KJ, Schmidt-Ott U, Kampmeier GE, Thompson FC, Grimaldi DA, Beckenbach AT, Courtney GW, Friedrich M, Meier R, Yeates DK: Episodic radiations in the fly tree of life. Proc Natl Acad Sci U S A 2011,108(14):5690–5695. 10.1073/pnas.1012675108View ArticleGoogle Scholar
- Xia X: DAMBE5: a comprehensive software package for data analysis in molecular biology and evolution. Mol Biol Evol 2013, 30: 1720–1728. 10.1093/molbev/mst064View ArticleGoogle Scholar
- Xia XH, Xie Z, Salemi M, Chen L, Wang Y: An index of substitution saturation and its application. Mol Phylogenet Evol 2003, 26: 1–7. 10.1016/S1055-7903(02)00326-3View ArticleGoogle Scholar
- Yang Z, Nielsen R, Goldman N, Pedersen AM: Codon-substitution models for heterogeneous selection pressure at amino acid sites. Genetics 2000,155(1):431–449.Google Scholar
- Yap VB, Lindsay H, Easteal S, Huttley G: Estimates of the effect of natural selection on protein-coding content. Mol Biol Evol 2010, 27: 726–734. 10.1093/molbev/msp232View ArticleGoogle Scholar
- Zaitzev VF: Ulidiidae (Diptera) in the Fauna of Mongolia. In Insects of Mongolia. Edited by: Kerzhner IM. Nauka, Leningrad; 1982:422–453.Google Scholar
- Zaitzev VF: Family Ulidiidae. In Catalogue of Palaearctic Diptera. Edited by: Soós A, Papp L. Akadémiai Kiádo. Elsevier, Amsterdam; 1984:59–66.Google Scholar
- Zhang B, Liu YH, Wu WX, Wang ZL: Molecular phylogeny of Bactrocera species (Diptera: Tephritidae: Dacini) inferred from mitochondrial sequences of 16S rDNA and COI sequences. Fla Entomol 2010,93(3):369–377. 10.1653/024.093.0308View ArticleGoogle Scholar
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