Table 2 Proteins from ER, Golgi network and plasma membrane
From: Proteomic study of the brackish water mussel Mytilopsis leucophaeata
Protein | Pro-/Eucaryote | Function | Localisation |
|---|---|---|---|
Endoplasmin [O18750] | Oryctolagus cuniculus (rabbit) | Ca2+-binding protein, possibly involved in protein folding (Rowling et al. 1994) | ER |
Guanine nucleotide-binding protein subunit β [Q5GIS3] | Pinctada fucata (Japanese pearl oyster) | Cargo transport from trans-Golgi network to plasma membrane | Golgi network and plasmalemma (Irannejad and Wedegaertner 2010) |
AP-1 complex, subunit β-1 [O35643] | Mus musculus (mouse) | Subunit of adaptor protein complex-1, involved in protein sorting, mediating the recruitment of clathrin to the membrane and recognition of sorting signals within the cytoplasmic tails of transmembrane cargo molecules | Trans-Golgi network and/or clathrin-coated vesicles (UniProt KB/Swiss- Prot:O35643.2, cf. Robinson and Bonifacino 2001) |
Clathrin heavy chain 1 [P11442] | Rattus norvegicus | Involved in cargo sorting (cf. adaptor protein complex-1 of Mus musculus) | Clathrin-coated vesicles at the plasma membrane or trans-Golgi network |
α-Amylase [P04745] | Homo sapiens (human) | Formation of maltose, maltotriose and α-dextrin from starch | In oyster (Crassostrea gigas) preferentially in digestive tract (Huvet et al. 2003), RER, Golgi, cisternae, condensing vacuoles, and secretory granules (Geuze et al. 1979) |
Glyceraldehyde-3-phosphate dehydrogenase 2 [Q9ESV6] | Rattus norvegicus | Conversion of glyceraldehyde-3P to 1,3-bisphosphoglycerate; + Rab2 and protein kinase Ci driven tubulovesicular recycling of proteins from the Golgi to the ER (Tisdale et al. 2009) | Vesicular tubular clusters |
Transitional endoplasmic reticulum ATPase (TERA) [P03974] | Sus scrofa (wild boar) | Involved in fragmentation of Golgi stacks during mitosis and reassembly after mitosis; further is TERA involved in the formation of tER (transitional ER) (UniProt KB/Swiss-Prot information) | Golgi and ER |
Guanine nucleotide-binding protein G(o), subunit α [O15976] | Mizuhopecten yessoensis (Yesso scallop) | Major neural signalling GTPase. Reacts on food deprivation (Hofler and Koelle 2011) |  |
Idem G(q), subunit α [P38411] | Lymnaea stagnalis (great pond snail) |  |  |
Guanine nucleotide-binding protein subunit β-2-like 1, Rack1 = receptor for activated c kinase-1 [Q93134] | Biomphalaria glabrata (blood fluke planorb = gastropod) | Involved in integrin signalling at adhesions, e.g. in a complex with kindlin-3 | Plasmalemma (Feng et al. 2012) |
Peptidyl-prolyl cis-trans isomerase C (cyclophilin C) [Q08E11] | Bos taurus (cow) | Cyclophilin is a protein folding catalyst | ER (Wang and Heitman 2005) |
Actin, cytoplasmic 2 (from fibroblastic and epithelial cells) [A2BDB0] | Xenopus laevis (African clawed frog) | Probably involved in contractile ring formation (Dugina et al. 2009) | Colocalisation with myosin 2a in stress fibres and with VASP (vasodilator-stimulated phosphoprotein) in lamellipodia and focal adhesions (Dugina et al. 2009) |
ADP-ribosylation factor 1 (Arf 1) [P36579] | Schizosacharomyces pombe 972 h- | Protein trafficking via COPI or clathrin-coated vesicles from ER to Golgi and plasmalemma | Cis/trans-Golgi and plasmalemma (Chavrier and Goud 1999; D’Souza-Schorey and Chavrier 2006) |
Peptidoglycan-associated lipoprotein [P0A138] | Pseudomonas putida KT2440 | Presence in bivalves may be due to ingestion by the host (Wood 2011) | Cell outer membrane |
Sarcoplasmic/endoplasmic reticulum calcium ATPase 3 [Q9YGL9] | Gallus gallus (chicken) | Involved in muscle contraction | Localisation is in the name |
Outer membrane porin F [P37726] | Pseudomonas fluorescens | Stabilisation of plasmalemma (multipass membrane protein) | Cell outer membrane (cf. VDAC in C. anguineus, Schuurmans Stekhoven et al. 2012) |
Ribulose bisphosphate carboxylase large chain [A1EA16] | Agrostis stolonifera (creeping bent grass) | Ribulose 1,5-bisphosphate + CO2 + H2O → 2 3-P-glycerate + 2H+, and 3-P-glycerate + 2-P-glycolate → ribulose 1,5-bisP + O2 | Reactions take place in chloroplasts; presence in mollusks indicates contamination by plants |
Glycogen phosphorylase from liver [P06737] | Homo sapiens (human) | Glycogen (n) + Pi↔glucose-1P + glycogen(n-1) | Microsomal fraction (ER or glycogen particles) (Tata 1964, Margolis et al. 1979) |
Heat shock 70 kDa protein [P08106] | Gallus gallus (chicken) | Conservation of protein shape and anti-stress protectant (De Maio 1999) | Cytosol, plasma membrane and endosomes + lysosomes (Nylandsted et al. 2004) |
Heat shock 70Â kDa protein cognate 3 [P29844] | Drosophila melanogaster (fruit fly) | Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER | ER (for additional locations and actions see above) |
78 kDa glucose-regulated protein [Q16956] | Aplysia californica (California sea hare = mollusc) | Belongs to the heat shock protein 70 family with function as above | ER |
Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit STT3A [P46977] | Homo sapiens (human) | Transfer of a high mannose oligosaccharide to Asn-X-Ser/Thr in nascent polypeptide chains. The complex associates with Sec61 at the channel forming translocon complex for protein translocation across the ER | ER (expressed at high levels in the placenta, liver, muscle, pancreas; low in the brain, lung, and kidney) |
Calcium transporting ATPase [P22700] | Drosophila melanogaster (fruit fly) | Reversible Ca2+ transport | Via ER and plasmalemma |
Trypsin [P00761] | Sus scrofa (wild boar) | Serine protease | In digestive tract (synthesised in pancreas), localised in plasmalemma (Takeuchi et al. 2000) |
Na+/K+-ATPase, α-subunit [P05025] | Torpedo californica (Pacific electric ray) | 3Na+/2 K+ exchange | Plasmalemma |
Gelsolin-like protein 1 [Q7JQD3] | Lumbricus terrestris (common earth worm) | Regulator of actin filament assembly and disassembly | Plasma and intracellular membranes, including ER, cortical vesicles and mitochondria, plus short actin filaments adhering to the plasma membrane (Hartwig et al. 1989) renal brush border membranes |
Malate dehydrogenase cytoplasmic [Q6PAB3] | Xenopus laevis (African clawed frog) | Oxidation of malate to oxaloacetate; confers selectivity of the nucleic acid-conducting channel in renal brush border membranes (Hanss et al. 2002) | Renal brush border membranes |
Guanine nucleotide- binding protein subunit β-1 [P17343] | Caenorhabditis elegans | Involved in Gαβγ activation of phospholipase c activity in a cellular signalling process (Yan et al. 2007) | Localisation not disclosed |
Ras-related protein O-Rab1 [P22125] | Discopyge ommata (ocellated electric ray) | Probably involved in vesicular traffic | O-Rab1 was found largely in the synaptic vesicle fraction (Ngsee et al. 1991) |
Enolase [O02654] | Loligo pealei (longfin inshore squid) | Conversion of 2-phosphoglycerate ↔ phosphenolpyruvate + H2O | Cytoplasm and plasma membrane of synaptosomes (Ueta et al. 2004) |
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [B6IYD3] | Rhodospirillum centenum SW | Catalyses the interconversion of 2-phosphoglycerate and 3-phosphoglycerate | Â |
Ras-related protein Rap-1b [A5A6J7] | Pan troglodytes (chimpanzee) | Ca2+ ATPase effector (Lacabaratz-Porret et al. 1998) | Plasmalemma (Marridonneau-Parini and de Gunzburg 1992; Mollinedo et al. 1993) |