Reverse FISH analysis of microdissected centromeric DNA. The DOP-PCR products from the microdissected DNA of pericentromeric/centromeric region of chromosome X + 3 were labeled with DIG-dUTP, hybridized to male Indian muntjac chromosome spreads and detected with FITC-conjugated anti-DIG antibody (green). The centromeric heterochromatin regions of all chromosomes show positive FITC hybridization signals. Chromosomes were counterstained with DAPI (blue). Scale bar = 10 μm.