Dot-blot analysis of the levels of ubiquitin-conjugated proteins in the gills of blue-green damselfish ( Chromis viridis ). (A) Ubiquitin-conjugated protein levels were shown as relative values based on dot intensities. (B) Ponceau S total protein stain of blots was used as loading control. (C) Significant elevation of the levels of ubiquitin-conjugated proteins was found after hyperthermic exposure. Unpaired t test was used for the statistics. Values are means ± S.E.M (n = 5). NT, normal temperature (26°C); HT, high temperature (32°C).