Protein expression of branchial heat shock protein 90 (HSP90), HSP70, and HSP60 in blue-green damselfish ( Chromis viridis ). The representative immunoblots of HSP90, HSP70, and HSP60 showed a single immunoreactive band with a molecular mass about 90 (A), 70 (C), and 60 kDa (E), respectively. The immunoreactive bands of HT group are more intensive than that in the NT group. The protein amounts of HSP90 (B), HSP70 (D), and HSP60 (F) increased evidently after hyperthermic exposure (n = 5). β-actin was used as the loading control. The asterisk indicated a significant difference (P < 0.05) by unpaired t test. Values were mean ± SEM. M, marker; NT, normal temperature (26°C); HT, high temperature (32°C).