Table 1 EST-4 purification features from Drosophila mulleri and Drosophila arizonae after MMEC and IEC
Purification steps | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Recovery (%) | Purification (fold) |
|---|---|---|---|---|---|
D. mulleri | |||||
Crude extract | 0.1400 | 46.00 | 328.57 | 100.00 | 1.00 |
MMEC | 0.0030 | 2.38 | 793.33 | 5.17 | 2.41 |
IEC | 0.0015 | 1.66 | 1,106.66 | 3.61 | 3.37 |
D. arizonae | |||||
Crude extract | 0.1700 | 241.35 | 1,419.70 | 100.00 | 1.00 |
MMEC | 0.0530 | 126.00 | 2,377.36 | 52.20 | 1.67 |
IEC | 0.0036 | 16.05 | 4,458.33 | 6.65 | 3.14 |