PCR products of various toads and frogs (A), and digestion of Bam HI (B). B. bankorensis (Bu), B. melanostictus (BM), B. gargarizans (BG), Buergeria robusta (B), and R. swinhoana (S). Buergeria robusta (B) and R. swinhoana (S) were used to test the specificity of the primer set. No specific bands were found for B. melanostictus or R. swinhoana, and no further sequencing was performed. Bu1 and Bu3 are the eastern clade. Bu2 is the western clade. Bu1, Bu2, Bu3, BM1, BM2, BM3, B1, B2, B3, S1, S2, S3, BG1, BG2, BG3, and BG4 represent different individual samples in each group.