Low phylogeographic structure of Rhinella arunco (Anura: Bufonidae), an endemic amphibian from the Chilean Mediterranean hotspot

Zoological Studies

Table 2 Geographic distributions of the 67 haplotypes of the mitochondrial control region of R. arunco and measures of intrapopulational genetic variation

Number	Locality (abbreviation)	Number of sequences	Number of haplotypes	Haplotype diversity ± SD	Π ± SD	Haplotypes
1	Quilimarí (Qui)	14	6	0.802 ± 0.090	2.450 ± 1.409	1-6
2	Los Molles (LM)	5	3	0.800 ± 0.164	1.800 ± 1.236	2, 7, 8
3	El Trapiche (ET)	10	8	0.956 ± 0.059	5.555 ± 2.915	2, 4, 9-14
4	Illalolén^a (Illa)	3	1			15
5	Campos de Ahumada Bajo^a (CAB)	3	2			9, 16
6	Estero Marga Marga (MM)	6	3	0.733 ± 0.155	9.200 ± 4.937	17-19
7	Estero Puangue (EP)	10	8	0.933 ± 0.077	7.356 ± 3.761	2, 20-26
8	Río Molina (RM)	4	2	0.500 ± 0.265	5.500 ± 3.343	27, 28
9	Quebrada de Córdova (QC)	11	5	0.818 ± 0.083	3.018 ± 1.703	1, 17, 22, 29, 30
10	Estero Ñanco (EÑ)	5	4	0.900 ± 0.161	9.200 ± 5.106	31-34
11	Estero Coyanco (EC)	12	7	0.909 ± 0.056	8.393 ± 4.181	24, 27, 33, 35-38
12	Topocalma (Top)	6	4	0.867 ± 0.129	1.933 ± 1.269	39-42
13	Pumanque (Pmq)	7	4	0.809 ± 0.130	2.190 ± 1.372	43-46
14	Sierras de Bellavista (SB)	13	4	0.654 ± 0.106	3.307 ± 1.816	27, 47-49
15	Pumaitén (Pum)	4	2	0.667 ± 0.204	0.666 ± 0.626	39, 50
16	Linares de Perales (LiPe)	4	3	0.833 ± 0.222	5.500 ± 3.343	51-53
17	La Suiza (LS)	7	2	0.286 ± 0.196	1.428 ± 0.984	54, 55
18	Vega de Salas^a (VS)	2	2			56, 57
19	La Raya (LR)	8	5	0.857 ± 0.108	5.750 ± 3.079	58-62
20	Manzanares (Man)	10	5	0.844 ± 0.080	3.555 ± 1.972	55, 60, 63-65
21	Río Ñuble (RÑ)	4	1	0	0	60
22	Las Juntas de Nahuelbuta (LJN)	4	1	0	0	66
23	Río Bureo (RB)	8	2	0.536 ± 0.123	0.535 ± 0.492	60, 67

Geographic distributions of the 67 haplotypes of the mitochondrial control region found in R. arunco and measures of intrapopulational genetic variation by locality (number of haplotypes, haplotype diversity, and mean differences between sequence pairs (Π)). Figure 2 shows relationships between haplotypes as inferred by the median-joining method. ^aLocalities with fewer than four individuals. SD, standard deviation.