DNA variations of the green toad Pseudepidalea viridis (syn. Bufo viridis) from various habitats

Zoological Studies

Table 7 AMOVA for individuals of P. viridis from breeding sites based on RAPD and AFLP analyses

Site part		df	SS	MS	Est. var.	Total variance (%)	Phi	p value
(A) Israeli sites: RAPD	Among regions	2	13.46	6.73	0.09	4	PhiRT 0.04	0.010
	Among populations	18	77.0	4.33	0.80	34	PhiPR 0.36	0.010
	Within populations	55	79.83	1.45	1.45	62	PhiPT 0.38	0.010
(B) Israeli sites: AFLP	Among regions	2	86.74	43.37	0.53	2	PhiRT 0.02	0.010
	Among populations	17	523.20	30.78	2.06	8	PhiPR 0.08	0.010
	Within populations	52	1217.0	23.40	23.40	90	PhiPT 0.10	0.010
(C) Israeli sites + 4 out-group populations: AFLP	Among regions	3	37.79	12.60	0.56	69	PhiRT 0.69	0.010
(C) Israeli sites + 4 out-group populations: AFLP	Among populations	20	17.83	0.89	0.24	30	PhiPR 0.96	0.010
	Within populations	62	0.58	0.01	0.01	1	PhiPT 0.99	0.010

AMOVA for individuals of P. viridis from breeding sites in Israel (parts A and B) and from four sites in other countries (part C) based on RAPD and AFLP analyses. p value estimates are based on 999 permutations. df degrees of freedom, SS sum of squared observations, MS mean of squared observations, Est var estimated variance, PhiPT proportion of the total genetic variance among individuals within populations, PhiRT proportion of the total genetic variance among regions, PhiPR proportion of the total genetic variance among populations within regions.