DNA variations of the green toad Pseudepidalea viridis (syn. Bufo viridis) from various habitats

Zoological Studies

Table 5 AMOVA for individuals of P. viridis from breeding sites based on D-loop and Cyt b fragments

Site part		df	SS	MS	Est. var.	Total variance (%)	Phi	p value	Nm
(A) Israeli sites: control region	Among regions	2	4.37	2.18	0.0	0	PhiRT 0.01	0.800	1.459
	Among populations	19	51.01	2.69	0.39	26	PhiPR 0.27	0.010
	Within populations	70	75.13	1.07	1.07	74	PhiPT 0.26	0.010
(B) Israeli sites: Cyt b	Among regions	2	0.90	0.45	0.0	0	PhiRT 0.09	1.000	0.039
	Among populations	19	18.77	0.99	0.22	93	PhiPR 0.93	0.010
	Within populations	76	1.20	0.02	0.02	7	PhiPT 0.92	0.010
(C) Israeli sites + 4 out-group populations: control region	Among regions	6	357.36	59.56	4.70	73	PhiRT 0.73	0.010	0.143
	Among populations	19	51.01	2.68	0.30	5	PhiPR 0.17	0.020
	Within populations	79	112.87	1.43	1.43	22	PhiPT 0.78	0.010
(D) Israeli sites + 4 out-group populations: Cyt b	Among regions	6	58.81	9.80	0.65	72	PhiRT 0.72	0.010	0.024
(D) Israeli sites + 4 out-group populations: Cyt b	Among populations	19	18.77	0.99	0.21	24	PhiPR 0.84	0.010	0.024
	Within populations	89	3.60	0.04	0.04	4	PhiPT 0.96	0.010

AMOVA for individuals of P. viridis from breeding sites in Israel (parts A and B) and from four sites in other countries (parts C and D), based on the control region and Cyt b fragments. p value estimates were based on 999 permutations. df, degrees of freedom; SS, sum of squared observations; MS, mean of squared observations; Est. var., estimated variance; PhiPT, proportion of the total genetic variance among individuals within populations; PhiRT, proportion of the total genetic variance among regions; PhiPR, proportion of the total genetic variance among populations within regions.