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Table 5 AMOVA for individuals of P. viridis from breeding sites based on D-loop and Cyt b fragments

From: DNA variations of the green toad Pseudepidalea viridis (syn. Bufo viridis) from various habitats

Site part df SS MS Est. var. Total variance (%) Phi p value Nm
(A) Israeli sites: control region Among regions 2 4.37 2.18 0.0 0 PhiRT 0.01 0.800 1.459
Among populations 19 51.01 2.69 0.39 26 PhiPR 0.27 0.010
Within populations 70 75.13 1.07 1.07 74 PhiPT 0.26 0.010
(B) Israeli sites: Cyt b Among regions 2 0.90 0.45 0.0 0 PhiRT 0.09 1.000 0.039
Among populations 19 18.77 0.99 0.22 93 PhiPR 0.93 0.010
Within populations 76 1.20 0.02 0.02 7 PhiPT 0.92 0.010
(C) Israeli sites + 4 out-group populations: control region Among regions 6 357.36 59.56 4.70 73 PhiRT 0.73 0.010 0.143
Among populations 19 51.01 2.68 0.30 5 PhiPR 0.17 0.020
Within populations 79 112.87 1.43 1.43 22 PhiPT 0.78 0.010
(D) Israeli sites + 4 out-group populations: Cyt b Among regions 6 58.81 9.80 0.65 72 PhiRT 0.72 0.010 0.024
Among populations 19 18.77 0.99 0.21 24 PhiPR 0.84 0.010
  Within populations 89 3.60 0.04 0.04 4 PhiPT 0.96 0.010  
  1. AMOVA for individuals of P. viridis from breeding sites in Israel (parts A and B) and from four sites in other countries (parts C and D), based on the control region and Cyt b fragments. p value estimates were based on 999 permutations. df, degrees of freedom; SS, sum of squared observations; MS, mean of squared observations; Est. var., estimated variance; PhiPT, proportion of the total genetic variance among individuals within populations; PhiRT, proportion of the total genetic variance among regions; PhiPR, proportion of the total genetic variance among populations within regions.