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Zoological Studies

Table 1 Differentially expressed proteins between 2u and Amn X8 flies by MALDI-TOF peptide mass fingerprinting after 2D-DIGE

From: Comparative proteomics analysis of normal and memory-deficient Drosophila melanogaster heads

Spot number

Protein name

Theoretical MW (Da)/pI

Observe MW (Da)/pI

Score

Queries matched

Coverage (%)

112

Drosocrystallin

55,248/5.54

130,000/5.5

90

12

40

285

Transferrin precursor

72,964/6.69

90,000/6.7

161

15

31

297

Tropomyosin-1, isoforms 33/34

54,666/4.77

90,000/4.5

79

13

38

300

Tropomyosin-2

32,961/4.39

40,000/4.8

122

19

25

324

Heat shock 70-kDa protein cognate 4

71,372/5.36

72,000/5.5

83

12

26

351

Phosphoenolpyruvate carboxykinase (GTP)

71,482/7.50

71,000/7.0

142

19

31

363

Esterase-6

61,486/5.57

68,000/5.7

233

21

48

408

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial

72,868/6.65

66,000/6.3

108

27

48

513

Actin

42,072/5.29

58,000/5.5

112

11

43

562

Actin

42,072/5.29

55,000/5.3

67

10

30

571

Actin

42,072/5.29

55,000/5.5

80

10

37

642

Enolase

54,561/8.68

54,500/6.3

134

13

39

675

CG2233-PA (GH20802p)

47,107/5.95

47,000/5.9

167

18

48

679

Gene P1 protein

119,350/5.82

47,000/6.4

81

14

18

711

Vitellogenin II precursor

49,762/7.74

46,500/6.4

90

14

49

716

Vitellogenin I precursor

48,739/7.19

46,500/5.9

134

20

41

740

Isocitrate dehydrogenase, isoform D

46,729/5.93

46,000/6.4

54

10

28

750

Actin

42,072/5.29

45,000/5.3

95

10

34

958

Aldehyde reductase

36,185/6.21

36,000/6.2

154

14

61

959

CG9119-PA (GH07301p)

35,866/5.96

39,000/6.0

83

9

40

982

Glycerol-3-phosphate dehydrogenase

39,627/6.33

38,500/6.5

84

11

36

989

Glycerol-3-phosphate dehydrogenase

39,627/6.33

38,500/6.8

84

16

36

994

Glycerol-3-phosphate dehydrogenase

39,627/6.33

38,500/6.5

72

13

38

1000

Glycerol-3-phosphate dehydrogenase

39,627/6.33

38,500/6.8

87

12

44

1017

IP10437p (Fragment)

36,568/6.79

38,500/7.5

138

12

46

1029

Probable transaldolase

36,939/7.55

38,000/6.0

120

15

47

1139

14-3-3 protein

28,210/4.77

29,000/4.7

61

13

38

1234

Ferritin 2 light chain homolog

25,489/5.90

26,000/6.3

183

14

63

1380

Cofilin/actin-depolymerizing factor homolog

17,428/6.74

20,000/5.8

126

14

70

1502

Proteasome endopeptidase complex

26,004/6.21

26,000/5.5

93

9

43

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