Changes in the differential expression and statistical value between 2u and AmnX8
flies. Measured values were normalized against the Cy2-labeled internal standard. Images of 2u and AmnX8 flies were respectively labeled with Cy3 and Cy5. The selected area of 2D-DIGE (marked in purple) and corresponding spot volumes were analyzed with DeCyder vers. 7.0 software. The statistical parameters used in this experiment were set to a p value of ≤0.05, and the average ratio was beyond ±1.5. (A) Fifteen proteins were upregulated (15 spots) in 2u flies. (B) Fifteen proteins were downregulated (15 spots) in AmnX8 flies.