Superimposed 2D-DIGE image of 2u and AmnX8
flies. (A) Head proteins of Drosophila melanogaster extracted from wild-type 2u flies (left) and AmnX8 mutant flies (right) were analyzed by 2D-DIGE. The pH range and molecular weight marker are indicated in the figures. (B) Images shown in (A) were superimposed. Differentially expressed protein features are marked in white circles, and their corresponding identification and information are given in Table 1. Pink spots represent upregulation of protein expression in wild-type 2u flies. Green spots represent upregulation of protein expression in AmnX8 flies. Yellow spots represent an equal amount of protein expression in both the wild-type 2u and AmnX8 strains.