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Table 1 Digestive enzyme assays used in the experiment

From: Effects of sex on characteristics and expression levels of digestive enzymes in the adult guppy Poecilia reticulata

Digestive enzyme

Substrate

Reference

α-Amylase (EC 3.2.1.1)

Soluble starch

Areekijseree et al. (2004)

Lipase (EC 3.1.1.3)

p-Nitrophenyl palmitate

Winkler and Stuckmann (1979)

Total proteases

Azocasein

Areekijseree et al. (2004)

Trypsin (EC 3.4.21.4)

n-Benzoyl-l-arginine-p-nitroanilide

Rungruangsak-Torrissen et al. (2006)

Chymotrypsin (EC 3.4.21.1)

n-Succinyl-ala-ala-pro-phenylalanine-p-nitroanilide

Rungruangsak-Torrissen et al. (2006)